Inactivation of dengue virus in plasma with amotosalen and ultraviolet A illumination. Issue 11 (20th May 2014)

• Record Type: Journal Article
• Authors: Musso, Didier; Richard, Vaea; Broult, Julien; Cao‐Lormeau, Van‐Mai
• Is Part Of: Transfusion. Volume 54:Issue 11(2014)
• Page Start: 2924
• Page End: 2930
• Publication Date: 2014-05-20
• Subjects: Hematology -- Periodicals; Blood -- Transfusion -- Periodicals; Blood Group Antigens -- Periodicals; Blood Preservation -- Periodicals; Blood Transfusion -- Periodicals; 615
• DOI: 10.1111/trf.12713
• Languages: English
• ISSNs: 0041-1132

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="trf12713-sec-0001" sec-type="section"> <title>Background</title> <p>Dengue virus (DENV) is the most prevalent arbovirus in tropical and subtropical regions. Transfusion‐transmitted DENV infections have already been reported and the risk for blood products to be contaminated by DENV needs to be considered in dengue‐endemic areas, especially during outbreaks. Blood product inactivation processes, including amotosalen and ultraviolet A (UVA) illumination, have been developed to reduce transfusion‐transmitted infections. In this study we demonstrate the efficiency of using amotosalen and UVA illumination for DENV inactivation in human plasma.</p> </sec> <sec id="trf12713-sec-0002" sec-type="section"> <title>Study Design and Methods</title> <p>Plasma units from volunteer blood donors were spiked with DENV. Viral titers and viral RNA loads were measured in plasma before and after amotosalen and UVA photochemical treatment.</p> </sec> <sec id="trf12713-sec-0003" sec-type="section"> <title>Results</title> <p>The mean DENV titer in plasma before inactivation was 5.61 log 50% tissue culture infectious dose (TCID<sub>50</sub>)/mL and the mean viral RNA load was 10.21 log copies/mL. In inactivated plasma, the mean DENV RNA load was 9.37 log copies/mL, but cell cultures inoculated with inactivated plasma did not result in infected cells and did not produce any replicative virus nor detectable viral RNA.</p> </sec> <sec id="trf12713-sec-0004" sec-type="section"> <title>Conclusion</title> <p>We report here that amotosalen combined with UVA light inactivated DENV in fresh‐frozen plasma (5.61 log inactivation of viral titer). This inactivation process is an efficient method to prevent plasma transfusion‐transmitted DENV infections.</p> </sec> </abstract>