Effect of proteases against biofilms of Staphylococcus aureus and Staphylococcus epidermidis. (23rd July 2014)

• Record Type: Journal Article
• Authors: Elchinger, P.‐H.; Delattre, C.; Faure, S.; Roy, O.; Badel, S.; Bernardi, T.; Taillefumier, C.; Michaud, P.
• Is Part Of: Letters in applied microbiology. Volume 59:Number 5(2014:Nov.)
• Page Start: 507
• Page End: 513
• Publication Date: 2014-07-23
• Subjects: Microbiology -- Periodicals; 660.62
• DOI: 10.1111/lam.12305
• Languages: English
• ISSNs: 0266-8254

Abstract

<abstract abstract-type="main" id="lam12305-abs-0001"> <title>Abstract</title> <sec id="lam12305-sec-1001" sec-type="section"> <p>Biofilms play a key role in bacterial resistance against antibacterial agents—an issue that causes multiple problems in medical fields, particularly with <italic>Staphylococcus</italic> biofilms that colonize medical indwelling devices. The literature reports several anti‐biofilm strategies that have been applied in medicine. Disrupting the biofilm formation process creates new sites open to colonization by treatment‐generated planktonic bacteria, so efforts have turned to focus on strategies to prevent and control the initial <italic>Staphylococci</italic> adhesion. Here, we investigated the preventive activities of three commercial proteases (Flavourzyme, Neutrase and Alcalase) against biofilm formation by two Staphylococcus strains. Some proteolytic extracts revealed interesting results with <italic>Staphylococcus epidermidis</italic> and <italic>Staphylococcus aureus aureus</italic> biofilms.</p> </sec> <sec id="lam12305-sec-1002" sec-type="section"> <title>Significance and Impact of the Study</title> <p>Three proteases were tested against <italic>Staphylococcus aureus</italic> and <italic>Staphylococcus epidermidis</italic> biofilms in standard conditions. The Flavourzyme containing a mix of <italic>Aspergillus orizae</italic> endo‐ and exoproteases demonstrated significant efficacy against <italic>Staph. epidermidis</italic> biofilm formation. These results could prove valuable in the effort to develop simple anti‐biofilm methods.</p> </sec> </abstract>