Tumor Necrosis Factor‐α and Interleukin‐6 Concentrations in Cerebrospinal Fluid of Dogs After Seizures. (10th October 2014)

• Record Type: Journal Article
• Authors: Merbl, Y.; Sommer, A.; Chai, O.; Aroch, I.; Zimmerman, G.; Friedman, A.; Soreq, H.; Shamir, M.H.
• Is Part Of: Journal of veterinary internal medicine. Volume 28:Number 6(2014:Nov./Dec.)
• Page Start: 1775
• Page End: 1781
• Publication Date: 2014-10-10
• Subjects: Veterinary medicine -- Periodicals; 636.0896
• DOI: 10.1111/jvim.12462
• Languages: English
• ISSNs: 0891-6640

Abstract

<abstract abstract-type="main" id="jvim12462-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="jvim12462-sec-0001" sec-type="section"> <title>Background</title> <p>Idiopathic and acquired epilepsy are common in dogs. Up to 30% of these dogs are refractory to pharmacological treatment. Accumulating experimental evidence indicates that brain immune response and presence of inflammatory mediators decrease the threshold for individual seizures and contribute to epileptogenesis.</p> </sec> <sec id="jvim12462-sec-0002" sec-type="section"> <title>Hypothesis</title> <p>Dogs with seizures have higher cerebrospinal interleukin‐6 (IL‐6) and tumor necrosis factor‐α (TNF‐α) concentrations compared to dogs with no seizures.</p> </sec> <sec id="jvim12462-sec-0003" sec-type="section"> <title>Methods</title> <p>A prospective double blinded study; cerebrospinal fluid (CSF) and serum IL‐6, TNF‐α and total protein (TP) concentrations were measured by a blinded investigator for the study group and CSF IL‐6 and TNF‐α levels and TP concentrations were measured in the control group (CG).</p> </sec> <sec id="jvim12462-sec-0004" sec-type="section"> <title>Animals</title> <p>Dogs presented with seizures that had enough CSF collected to allow analysis were included in the study group. Twelve apparently healthy, quarantined, stray dogs served as control (CG).</p> </sec> <sec id="jvim12462-sec-0005" sec-type="section"> <title>Results</title> <p>Cerebrospinal fluid TNF‐α and IL‐6 concentrations were significantly higher (<italic>P </italic>=<italic> </italic>.011, <italic>P </italic>=<italic> </italic>.039) in dogs with seizures (0 ± 70.66, 0.65 ± 10.93 pg/mL) compared to the CG (0 ± 19, 0.73 ± 0.55 pg/mL). When assessing cytokine concentrations of specifically the idiopathic epilepsy (IE) dogs compared to the CG, only TNF‐α concentrations (8.66 ± 62, 0 ± 19 pg/mL) were significantly higher (<italic>P </italic>=<italic> </italic>.01). CSF TP concentrations were not significantly higher in the study dogs compared to the CG.</p> </sec> <sec id="jvim12462-sec-0006" sec-type="section"> <title>Conclusions and Clinical Importance</title> <p>Higher TNF‐α and IL‐6 concentration in the CSF of dogs with naturally occurring seizures. The higher supports the hypothesis that inflammatory processes through certain mediators play a role in the pathogenesis of seizures in dogs.</p> </sec> </abstract>