Disease resistance and immune‐relevant gene expression in golden mandarin fish, Siniperca scherzeri Steindachner, infected with infectious spleen and kidney necrosis virus‐like agent. Issue 12 (24th September 2013)

• Record Type: Journal Article
• Authors: Shin, G W; White, S L; Dahms, H U; Jeong, H D; Kim, J H
• Is Part Of: Journal of fish diseases. Volume 37:Issue 12(2014:Dec.)
• Page Start: 1041
• Page End: 1054
• Publication Date: 2013-09-24
• Subjects: Fishes -- Diseases -- Periodicals; 639.3
• DOI: 10.1111/jfd.12182
• Languages: English
• ISSNs: 0140-7775

Abstract

<abstract abstract-type="main" id="jfd12182-abs-0001"> <title>Abstract</title> <p>Infectious spleen and kidney necrosis virus (ISKNV), family <italic>Iridoviridae</italic>, genus <italic>Megalocytivirus, </italic> may cause high mortality rates such as those seen in mandarin fish, <italic>Siniperca chuatsi</italic>. ISKNV has attracted much attention due to the possible environmental threat and economic losses it poses on both cultured and wild populations. We have investigated the pathogenicity of ISKNV‐like agent <italic>Megalocytivirus</italic>, isolated from infected pearl gourami, in golden mandarin fish, <italic>Siniperca scherzeri</italic> – a member of the <italic>Percichthyidae</italic> family – and in another <italic>Percichthyidae</italic> species, <italic>S. chuatsi</italic>. Fish were challenged with four different doses of ISKNV‐like agent <italic>Megalocytivirus</italic> (1, 10, 100 or 1000 μg per fish) over a 30‐day period, and cumulative fish mortalities were calculated for each group. No significant mortality was observed for fish challenged with the lowest dose (1 μg per fish) relative to a control group. However, all other challenged groups showed 100% mortality over a 30‐day period in proportion to the challenge dose. Quantitative real‐time PCR was performed to measure mRNA expression levels for six immune‐related genes in golden mandarin fish following ISKNV‐like agent challenge. mRNA expression levels for IRF1, Mx, viperin and interleukin 8 significantly increased, while mRNA levels for IRF2 and IRF7 remained constant or declined during the challenge period.</p> </abstract>