Evaluation of intra‐ and extra‐epithelial secretory IgA in chlamydial infections. Issue 4 (December 2014)

• Record Type: Journal Article
• Authors: Armitage, Charles W.; O'Meara, Connor P.; Harvie, Marina C. G.; Timms, Peter; Wijburg, Odilia L.; Beagley, Kenneth W.
• Is Part Of: Immunology. Volume 143:Issue 4(2014:Dec.)
• Page Start: 520
• Page End: 530
• Publication Date: 2014-12
• Subjects: Immunology -- Periodicals
• DOI: 10.1111/imm.12317
• Languages: English
• ISSNs: 0019-2805

Abstract

<abstract abstract-type="main" id="imm12317-abs-0001"> <title>Summary</title> <p>Immunoglobulin A is an important mucosal antibody that can neutralize mucosal pathogens by either preventing attachment to epithelia (immune exclusion) or alternatively inhibit intra‐epithelial replication following transcytosis by the polymeric immunoglobulin receptor (pIgR). <italic>Chlamydia trachomatis</italic> is a major human pathogen that initially targets the endocervical or urethral epithelium in women and men, respectively. As both tissues contain abundant secretory IgA (SIgA) we assessed the protection afforded by IgA targeting different chlamydial antigens expressed during the extra‐ and intra‐epithelial stages of infection. We developed an <italic>in vitro</italic> model using polarizing cells expressing the murine pIgR together with antigen‐specific mouse IgA, and an <italic>in vivo</italic> model using pIgR<sup>−/−</sup> mice. Secretory IgA targeting the extra‐epithelial chlamydial antigen, the major outer membrane protein, significantly reduced infection <italic>in vitro</italic> by 24% and <italic>in vivo</italic> by 44%. Conversely, pIgR‐mediated delivery of IgA targeting the intra‐epithelial inclusion membrane protein A bound to the inclusion but did not reduce infection <italic>in vitro</italic> or <italic>in vivo</italic>. Similarly, intra‐epithelial IgA targeting the secreted protease <italic>Chlamydia</italic> protease‐like activity factor also failed to reduce infection. Together, these data suggest the importance of pIgR‐mediated delivery of IgA targeting extra‐epithelial, but not intra‐epithelial, chlamydial antigens for protection against a genital tract infection.</p> </abstract>