Chitin deacetylase family genes in the brown planthopper, Nilaparvata lugens (Hemiptera: Delphacidae). Issue 6 (2nd July 2014)

• Record Type: Journal Article
• Authors: Xi, Y.; Pan, P.‐L.; Ye, Y.‐X.; Yu, B.; Zhang, C.‐X.
• Is Part Of: Insect molecular biology. Volume 23:Issue 6(2014:Dec.)
• Page Start: 695
• Page End: 705
• Publication Date: 2014-07-02
• Subjects: Insects -- Molecular aspects -- Periodicals; 595.7
• DOI: 10.1111/imb.12113
• Languages: English
• ISSNs: 0962-1075

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <p>Chitin deacetylases (CDAs) are enzymes required for one of the pathways of chitin degradation, in which chitosan is produced by the deacetylation of chitin. Bioinformatic investigations with genomic and transcriptomic databases identified four genes encoding CDAs in <italic>Nilaparvata lugens</italic> (<italic>NlCDA</italic>s). Phylogenetic analysis showed that insect <italic>CDA</italic>s were clustered into five major groups. Group I, III and IV <italic>CDA</italic>s are found in all insect species, whereas the pupa‐specific group II and gut‐specific group V <italic>CDA</italic>s are not found in the plant‐sap/blood‐sucking hemimetabolous species from Hemiptera and Anoplura. The developmental and tissue‐specific expression patterns of four <italic>NlCDA</italic>s revealed that <italic>NlCDA3</italic> was a gut‐specific <italic>CDA</italic>, with high expression at all developmental stages; <italic>NlCDA1</italic>, <italic>NlCDA2</italic> and <italic>NlCDA4</italic> were highly expressed in the integument and peaked periodically during every moulting, which suggests their roles in chitin turnover of the insect old cuticle. Lethal phenotypes of cuticle shedding failure and high mortality after the injection of double‐stranded RNAs (dsRNAs) for <italic>NlCDA1</italic>, <italic>NlCDA2</italic> and <italic>NlCDA4</italic> provide further evidence for their functions associated with moulting. No observable morphological and internal structural abnormality was obtained in insects treated with dsRNA for gut‐specific <italic>NlCDA3</italic>.</p> </abstract>