Stromal miR‐21 is more important than miR‐21 of tumour cells for the progression of gastric cancer. Issue 6 (2nd September 2014)

• Record Type: Journal Article
• Authors: Uozaki, Hiroshi; Morita, Shigeki; Kumagai, Arisa; Aso, Tatsuya; Soejima, Yurie; Takahashi, Yoshihisa; Fukusato, Toshio
• Is Part Of: Histopathology. Volume 65:Issue 6(2014)
• Page Start: 775
• Page End: 783
• Publication Date: 2014-09-02
• Subjects: Histology, Pathological -- Periodicals; 611.018
• DOI: 10.1111/his.12491
• Languages: English
• ISSNs: 0309-0167

Abstract

<abstract abstract-type="main" id="his12491-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="his12491-sec-0001" sec-type="section"> <title>Background</title> <p>Gastric cancer (GC) is a common cancer globally. miRNA‐21 (miR‐21) appears to be important in the tumourigenesis of almost all types of human cancer. However its precise localization in GC has yet to be clarified. We thus examined miR‐21 localization in GC and revealed its clinicopathological importance.</p> </sec> <sec id="his12491-sec-0002" sec-type="section"> <title>Methods</title> <p>Tissue arrays of 469 GCs from 454 patients were examined for miR‐21 using <italic>in situ</italic> hybridization (ISH). The positivity was evaluated separately in tumour cells and stromal cells. Conventional sections of 10 GCs were also stained. Eight cases were examined by quantitative RT‐PCR (qRT‐PCR).</p> </sec> <sec id="his12491-sec-0003" sec-type="section"> <title>Results</title> <p>miR‐21 was highly expressed in tumour cells of 44% of cases and in cancer stroma of 51% of cases. miR‐21 of tumour cells was not related to clinicopathological factors, whereas stromal miR‐21 was related to many factors including tumour stage, size, and nodal metastasis. Stromal miR‐21 gradually increased during tumour progression. ISH of whole sections showed stronger stromal positivity in invasive areas with desmoplastic reaction. Cancer stroma also showed higher miR‐21 expression than tumour and non‐tumourous tissue in the qRT‐PCR study.</p> </sec> <sec id="his12491-sec-0004" sec-type="section"> <title>Conclusion</title> <p>Stromal miR‐21 is closely related to tumour progression in GC. Stromal miR‐21 of tumours might be a target of treatment.</p> </sec> </abstract>