Transcriptional regulation of antimicrobial peptides in mucosal tissues of Atlantic cod Gadus morhua L. in response to different stimuli. Issue 12 (8th February 2013)

• Record Type: Journal Article
• Authors: Ruangsri, Jareeporn; Lokesh, Jep; Fernandes, Jorge M.O.; Kiron, Viswanath
• Is Part Of: Aquaculture research. Volume 45:Issue 12(2014:Nov.)
• Page Start: 1893
• Page End: 1905
• Publication Date: 2013-02-08
• Subjects: Aquaculture -- Periodicals; Fishery management -- Periodicals; 639.8
• DOI: 10.1111/are.12136
• Languages: English
• ISSNs: 1355-557X

Abstract

<abstract abstract-type="main" id="are12136-abs-0001"> <title>Abstract</title> <p>To understand the physiological roles of antimicrobial peptide (AMP) families in Atlantic cod, fish were subjected to abiotic (crowding/temperature rise) or biotic (commensal or pathogenic bacterium) stimuli, following which the transcriptional profiles of <italic>piscidin 1</italic> (<italic>pis1</italic>), <italic>piscidin 2</italic> (<italic>pis2</italic>), <italic>beta defensin</italic> (<italic>defb</italic>), <italic>hepcidin</italic> (<italic>hep</italic>) and <italic>cathelicidin 1</italic> (<italic>cath1</italic>) in selected mucosal tissues were analysed using qPCR. Unlike the crowding condition, the temperature change affected the transcripts of AMP genes – after the rise in temperature (0 h), <italic>cath1</italic> was downregulated mainly in gills, <italic>defb</italic> was downregulated in gills and skin, while <italic>hep</italic> was downregulated in gills, skin and rectum. These gene transcripts returned to their basal levels at 72 h, by which time <italic>pis1</italic> gene was upregulated in gills and skin. The <italic>pis2</italic> gene was apparently not altered by temperature change. Bath exposure to commensal bacterium affected only <italic>defb</italic> gene in gills (downregulated) and skin (upregulated). Bath challenge with live pathogen upregulated <italic>cath1</italic> gene mainly in gills, and <italic>hep</italic> gene in gills, skin and rectum, while <italic>pis1</italic> and <italic>pis2</italic> genes remained unaltered. The differential modulation of the AMP genes examined suggests that these peptides maintain distinct functions with respect to mucosal defence mechanisms in Atlantic cod.</p> </abstract>