Methionine restriction slows down senescence in human diploid fibroblasts. Issue 6 (1st October 2014)

• Record Type: Journal Article
• Authors: Kozieł, Rafał; Ruckenstuhl, Christoph; Albertini, Eva; Neuhaus, Michael; Netzberger, Christine; Bust, Maria; Madeo, Frank; Wiesner, Rudolf J.; Jansen‐Dürr, Pidder
• Is Part Of: Aging cell. Volume 13:Issue 6(2014:Dec.)
• Page Start: 1038
• Page End: 1048
• Publication Date: 2014-10-01
• Subjects: Cells -- Aging -- Periodicals; 571.8783605
• DOI: 10.1111/acel.12266
• Languages: English
• ISSNs: 1474-9718

Abstract

<abstract abstract-type="main" id="acel12266-abs-0001"> <title>Summary</title> <p>Methionine restriction (MetR) extends lifespan in animal models including rodents. Using human diploid fibroblasts (HDF), we report here that MetR significantly extends their replicative lifespan, thereby postponing cellular senescence. MetR significantly decreased activity of mitochondrial complex IV and diminished the accumulation of reactive oxygen species. Lifespan extension was accompanied by a significant decrease in the levels of subunits of mitochondrial complex IV, but also complex I, which was due to a decreased translation rate of several mtDNA‐encoded subunits. Together, these findings indicate that MetR slows down aging in human cells by modulating mitochondrial protein synthesis and respiratory chain assembly.</p> </abstract>