Insights into organohalide respiration and the versatile catabolism of Sulfurospirillum multivorans gained from comparative genomics and physiological studies. (3rd September 2014)

• Record Type: Journal Article
• Authors: Goris, Tobias; Schubert, Torsten; Gadkari, Jennifer; Wubet, Tesfaye; Tarkka, Mika; Buscot, Francois; Adrian, Lorenz; Diekert, Gabriele
• Is Part Of: Environmental microbiology. Volume 16:Number 11(2014:Nov.)
• Page Start: 3562
• Page End: 3580
• Publication Date: 2014-09-03
• Subjects: Microbial ecology -- Periodicals; Environmental Microbiology -- Periodicals; 579.17
• DOI: 10.1111/1462-2920.12589
• Languages: English
• ISSNs: 1462-2912

Abstract

<abstract abstract-type="main"> <title>Summary</title> <p> <italic>S</italic> <italic>ulfurospirillum multivorans</italic>, a free‐living ε‐proteobacterium, is among the best studied organisms capable of organohalide respiration. It is able to use several halogenated ethenes as terminal electron acceptor. In this report, the complete genome sequence of <italic>S</italic><italic>. multivorans</italic> including a comparison with genome sequences of two related non‐dehalogenating species, <italic>S</italic><italic>ulfurospirillum deleyianum</italic> and <italic>S</italic><italic>ulfurospirillum barnesii</italic>, is described. The 3.2 Mbp genome of <italic>S</italic><italic>. multivorans</italic> revealed a ∼ 50 kbp gene region encoding proteins required for organohalide respiration and corrinoid cofactor biosynthesis. This region includes genes for components not detected before in organohalide‐respiring organisms. A transcript analysis of genes coding for some of these proteins indicates the involvement of a putative quinol dehydrogenase in organohalide respiration. The presence of genes encoding a variety of oxidoreductases reflects the organism's metabolic versatility. This was confirmed by growth studies with different electron acceptors including perchlorate and several sulfur‐containing compounds. A comparison with other ε‐proteobacteria indicates horizontal acquisition of many genes in the <italic>S</italic><italic>. multivorans</italic> genome, which might be the basis of the bacterium's catabolic flexibility.</p> </abstract>