Identification of a novel cytochrome P450 gene, CYP321E1 from the diamondback moth, Plutella xylostella (L.) and RNA interference to evaluate its role in chlorantraniliprole resistance. Issue 6 (December 2014)

• Record Type: Journal Article
• Authors: Hu, Z.; Lin, Q.; Chen, H.; Li, Z.; Yin, F.; Feng, X.
• Is Part Of: Bulletin of entomological research. Volume 104:Issue 6(2014)
• Page Start: 716
• Page End: 723
• Publication Date: 2014-12
• Subjects: Entomology -- Periodicals; 595.7
• DOI: 10.1017/S0007485314000510
• Languages: English
• ISSNs: 0007-4853

Abstract

<abstract abstract-type="normal"> <title>Abstract</title> <p>Insect cytochrome P450 monooxygenases (P450s) play an important role in catalysis of many reactions leading to insecticides resistance. Our previous studies on transcriptome analysis of chlorantraniliprole-resistant development in the diamondback moth, <italic>Plutella xylostella</italic> revealed that up-regulation of cytochrome P450s are one of the main factors leading to the development of chlorantraniliprole resistance. Here, we report for the first time a novel cytochrome P450 gene <italic>CYP321E1</italic>, which belongs to the cytochrome P450 gene family CYP321. Real-time quantitative PCR (RT–qPCR) analyses indicated that <italic>CYP321E1</italic> was expressed at all developmental stages of <italic>P. xylostella</italic> but was highest in the fourth-instar larvae; furthermore, the relatively high expression was observed in the midgut of the fourth-instar larvae, followed by fat bodies and epidermis. The expression of <italic>CYP321E1</italic> in <italic>P. xylostella</italic> was differentially affected by three representative insecticides, including alphamethrin, abamectin and chlorantraniliprole. Among them, the exposure to chlorantraniliprole resulted in the largest transcript level of this cytochrome P450 gene. The findings suggested potential involvement of <italic>CYP321E1</italic> in chlorantraniliprole resistance of <italic>P. xylostella</italic>. To assess the functional link of <italic>CYP321E1</italic> to chlorantraniliprole resistance, RNA interference (RNAi)-mediated gene silencing by double stranded RNA (dsRNA) injecting was used. Results revealed that injection delivery of dsRNA can greatly reduce gene expression after 24 h. As a consequence of RNAi, a significant increment in mortality of larvae injected <italic>CYP321E1</italic> dsRNA was observed after 24 h of exposure to chlorantraniliprole. These results strongly support our notion that this novel cytochrome P450 gene plays an important role in chlorantraniliprole detoxification in the diamondback moth and is partly responsible for its resistance.</p> </abstract>