ToF‐SIMS analysis of diadenosine triphosphate and didadenosine tetraphosphate using bismuth and argon cluster ion beams. (10th April 2014)

• Record Type: Journal Article
• Authors: Shon, Hyun Kyong; Cho, Young‐Lai; Lim, Choung Su; Choi, Joon Sig; Chung, Sang J.; Lee, Tae Geol; Lee, Yeonhee; Moon, DaeWon; Kang, Hee Jae; Kim, Kyung Joong; Lee, Tae Geol; Lee, Jae Cheol; Yi, Keewook; Hong, Tae Eun
• Is Part Of: Surface and interface analysis. Volume 46:Number 1(2014:Jan.)
• Page Start: 189
• Page End: 192
• Publication Date: 2014-04-10
• Subjects: Surfaces (Physics) -- Periodicals; Surface chemistry -- Periodicals; Thin films -- Periodicals; 541.33
• DOI: 10.1002/sia.5514
• Languages: English
• ISSNs: 0142-2421

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>In the past few decades, research has shown diadenosine polyphosphates (Ap_nA) to be a family of intercellular and intracellular signaling molecules that play a key role in biological and pharmacological activities. ToF‐SIMS, recently shown to be a promising technique to analyze metabolites in biological samples due to its high sensitivity and specificity, was employed to analyze mixed diadenosine triphosphate (Ap₃A) and diadenosine tetraphosphate (Ap₄A) spiked in HeLa cell lysates by using bismuth and argon cluster ion beams. The molecular ion signals of Ap₃A and Ap₄A in the cell lysates were stronger when using an argon cluster ion beam than when using a bismuth cluster ion beam, although the detection limits of both molecules were the same. Using a correlation curve to indicate the molecular signal intensities and concentrations, the amount of Ap₃A and Ap₄A molecules in HeLa cell lysates could be quantified in the concentration range of 0.1 mM to 10 mM for Ap₃A, and 5 mM and 20 mM for Ap₄A. We believe that ToF‐SIMS analysis could be a powerful technique to detect important metabolite molecules such as Ap_nA for biomedical applications, without the need for a separation process. Copyright © 2014 John Wiley &amp; Sons, Ltd.</p> </abstract>