Enzymatic in situ saccharification of rice straw in aqueous‐ionic liquid media using encapsulated Trichoderma aureoviride cellulase. Issue 1 (4th July 2014)

• Record Type: Journal Article
• Authors: Xu, Jiaxing; Liu, Xiaoyan; He, Jianlong; Hu, Lei; Dai, Benlin; Wu, Bin
• Is Part Of: Journal of chemical technology & biotechnology. Volume 90:Issue 1(2015:Jan.)
• Page Start: 57
• Page End: 63
• Publication Date: 2014-07-04
• Subjects: Biotechnology -- Periodicals; Chemistry, Technical -- Periodicals; Chemical engineering -- Periodicals; Industries -- Environmental aspects -- Periodicals; 660
• DOI: 10.1002/jctb.4458
• Languages: English
• ISSNs: 0268-2575

Abstract

<abstract abstract-type="main" id="jctb4458-abs-0001"> <title>Abstract</title> <sec id="jctb4458-sec-0001" sec-type="section"> <title>BACKGROUND</title> <p id="jctb4458-para-0001">Enzymatic <italic>in situ</italic> saccharification of lignocellulose in ionic liquids (ILs) has become a hot topic of research, but is hampered by the incompatibility of ILs with cellulase. The aim of this present work was to improve IL tolerance of the cellulase from <italic>Trichoderma aureoviride</italic> strain HS through an efficient encapsulation method, and thus to develop a compatible IL–cellulase system for biorefining.</p> </sec> <sec id="jctb4458-sec-0002" sec-type="section"> <title>RESULTS</title> <p id="jctb4458-para-0002">The cellulase was encapsulated in alginate beads and its stability was greatly enhanced in various concentrations of 1‐ethyl‐3‐methylimidazolium dimethylphosphate ([Emim][DMP]). The encapsulated cellulase preserved 76% of the original activity in 40% (v/v) [Emim][DMP] for 12 h, whereas free cellulase lost nearly 95% activity. After treating rice straw with [Emim][DMP] at 100 °C and dilution to a final IL concentration of 40% (v/v), the pretreatment slurry was <italic>in situ</italic> hydrolyzed using encapsulated cellulase. Under the one‐pot process, a maximum saccharification rate of 84% was obtained, increased by 65% compared with that in buffer based on free cellulase.</p> </sec> <sec id="jctb4458-sec-0003" sec-type="section"> <title>CONCLUSION</title> <p id="jctb4458-para-0003">The hydrophilic alginate hydrogel protected the hydration shell of cellulase well against destruction by [Emim][DMP]. The feasibility of hydrolysis reaction in aqueous‐IL media using encapsulated cellulase was demonstrated, thus developing a one‐pot, wash‐free scheme for saccharification of lignocellulose. © 2014 Society of Chemical Industry</p> </sec> </abstract>