Ex vivo bone morphogenetic protein 2 gene delivery using periodontal ligament stem cells for enhanced re‐osseointegration in the regenerative treatment of peri‐implantitis. Issue 1 (18th March 2014)

• Record Type: Journal Article
• Authors: Park, Shin‐Young; Kim, Kyoung‐Hwa; Gwak, Eun‐Hye; Rhee, Sang‐Hoon; Lee, Jeong‐Cheol; Shin, Seung‐Yun; Koo, Ki‐Tae; Lee, Yong‐Moo; Seol, Yang‐Jo
• Is Part Of: Journal of biomedical materials research. Volume 103:Issue 1(2015:Jan.)
• Page Start: 38
• Page End: 47
• Publication Date: 2014-03-18
• Subjects: Biomedical materials -- Periodicals; 610.28
• DOI: 10.1002/jbm.a.35145
• Languages: English
• ISSNs: 1549-3296

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <p>Peri‐implantitis is a chronic inflammatory process with advanced bone loss and impaired healing potential. For peri‐implantitis treatment, tissue engineering can be applied to enhance bone regeneration of peri‐implant defects. This study aimed to evaluate <italic>ex vivo</italic> bone morphogenetic protein 2 (BMP2) gene delivery using canine periodontal ligament stem cells (PDLSCs) for regeneration of peri‐implantitis defects. Canine PDLSCs were transduced with adenoviral vectors containing BMP2 (BMP2/PDLSCs). After peri‐implantitis was induced by ligature placement in six beagle dogs, regenerative procedures were performed; hydroxyapatite (HA) particles and collagen gel with autologous canine PDLSCs (PDLSC group) or BMP2/PDLSCs (BMP/PDLSC group) or without cells (control group) were grafted into the defects and covered by an absorbable membrane. Three months later, the animals were sacrificed. <italic>In vitro</italic>, BMP2/PDLSCs showed similar levels of stem cell properties to PDLSCs, such as colony‐forming efficiency and expression of MSC markers STRO‐1 and CD 146. BMP2/PDLSCs produced BMP‐2 until day 21 at a concentration of 4–8 ng/mL. <italic>In vivo</italic>, the BMP2/PDLSC group showed significantly more new bone formation and re‐osseointegration in peri‐implantitis defects compared to the other groups. In conclusion, <italic>ex vivo</italic> BMP2 gene delivery using PDLSCs enhanced new bone formation and re‐osseointegration in peri‐implantitis defects. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 38–47, 2015.</p> </abstract>