MHC‐Ig induces memory T cell formation in vivo and inhibits tumour growth. Issue 3 (11th November 2014)

• Record Type: Journal Article
• Authors: Schütz, Christian; Zoso, Alessia; Peng, Shiwen; Bennett, Jonathon D.; Schneck, Jonathan P.; Oelke, Mathias
• Is Part Of: Immunity, inflammation and disease. Volume 2:Issue 3(2014)
• Page Start: 181
• Page End: 192
• Publication Date: 2014-11-11
• Subjects: Immunology -- Periodicals; Immunity -- Periodicals; Inflammation -- Periodicals; 616.079
• DOI: 10.1002/iid3.35
• Languages: English
• ISSNs: 2050-4527

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <sec id="iid335-sec-0001" sec-type="section"> <p>Induction of a T cell mediated immune response is critical for the eradication of viral infections and tumours. Soluble peptide‐loaded major histocompatibility complex‐Ig (^pep−MHC‐Ig) have been shown to bind their cognate ligands, T cell receptor, with high affinity, and are successfully used to visualize antigen‐specific T cells. Furthermore, immobilized ^pep−MHC‐Ig can activate and expand antigen‐specific T cells in vitro and <italic>in vivo</italic>. In this study, we investigate the use of ^pep−MHC‐Ig as a potential strategy to modulate antigen specific T cell immune responses <italic>in vivo</italic>. ^SIY−K^b‐Ig immunization, together with the pre‐activation by an anti‐CD40 monoclonal antibody, is able to stimulate a strong expansion of adoptively transferred 2C transgenic T cells and the formation of long term antigen‐specific memory T cells. In addition, mechanistic studies show that the ^pep−MHC‐Ig molecules directly activate T cells <italic>in vivo</italic> without requiring uptake and reprocessing by antigen‐presenting cells. Furthermore, B6 mice immunized with ^pep−MHC‐Ig molecules inhibit tumour growth in a B16‐SIY melanoma prevention model. Thus, soluble ^pep−MHC‐Ig molecules represent a powerful tool for active immunotherapy.</p> </sec> </abstract>