Efficient pretreatment for bioethanol production from water hyacinth (eichhornia crassipes) involving naturally isolated and recombinant enzymes and its recovery. Issue 4 (7th November 2013)

• Record Type: Journal Article
• Authors: Das, Saprativ P.; Ravindran, Rajeev; Ghosh, Arabinda; Deka, Deepmoni; Das, Debasish; Jawed, Mohammad; Fontes, Carlos M.G.A.; Goyal, Arun
• Is Part Of: Environmental progress & sustainable energy. Volume 33:Issue 4(2014:Dec.)
• Page Start: 1396
• Page End: 1404
• Publication Date: 2013-11-07
• Subjects: Environmental engineering -- Periodicals; Sustainable engineering -- Periodicals; Environmental chemistry -- Periodicals; 333.7
• DOI: 10.1002/ep.11885
• Languages: English
• ISSNs: 1944-7442

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>Simultaneous saccharification and fermentation (SSF) experiments involving water hyacinth (<italic>Eichhornia crassipes</italic>), an abundantly available renewable bioresource, using hydrolytic enzymes, and fermentative microbes were investigated. Water hyacinth containing 30.01 (%, w/w) cellulose, 44.49 (%, w/w) hemicellulose, and 20.04 (%, w/w) lignin was subjected to three different pretreatments, namely, wet oxidation, phosphoric acid (H<sub>3</sub>PO<sub>4</sub>)‐acetone, and ammonia fiber explosion (AFEX). Hydrolytic enzymes, namely, recombinant <italic>Clostridium thermocellum</italic> cellulase (GH5) and hemicellulase (GH43), <italic>Trichoderma reesei</italic> and <italic>Bacillus subtilis</italic> AS3 cellulases were employed separately for saccharification. <italic>Saccharomyces cerevisiae</italic> and <italic>Candida shehatae</italic> were used for fermentation. The AFEX pretreated 1% (w/v) water hyacinth along with recombinant cellulase (GH5)‐hemicellulase (GH43) consortium gave the highest ethanol titer of 1.52 g/L as compared with wet oxidation (1.23 g/L) and phosphoric acid‐acetone pretreatments (1.31 g/L). The best SSF combination with 5% (w/v) substrate at shake flask contributed an ethanol titer and yield of 7.83 g/L, 0.266 (g of ethanol/g of substrate) and its scale up at bioreactor level resulted in significantly higher ethanol titer and yield of 14.39 g/L and 0.489 (g/g), respectively. 93.0 (%, v/v) ethanol from bioreactor was recovered by rotary evaporator with 20.4% purification efficiency. © 2013 American Institute of Chemical Engineers Environ Prog, 33: 1396–1404, 2014</p> </abstract>