Reduced CD200 and CD200R1 expression in human chorionic villi contributes to early spontaneous abortion. (23rd September 2014)

• Record Type: Journal Article
• Authors: Wang, Li‐Qin; Yan, Chun‐Fang; Zhao, Yue; Chu, Jing; Yu, Xue‐Wen
• Is Part Of: Acta obstetricia et gynecologica Scandinavica. Volume 93:Number 12(2014)
• Page Start: 1248
• Page End: 1254
• Publication Date: 2014-09-23
• Subjects: Gynecology -- Periodicals; Pregnancy -- Periodicals; Obstetrics -- Periodicals; 618.05
• DOI: 10.1111/aogs.12476
• Languages: English
• ISSNs: 0001-6349

Abstract

<abstract abstract-type="main" id="aogs12476-abs-0001"> <title>Abstract</title> <sec id="aogs12476-sec-0001" sec-type="section"> <title>Objective</title> <p>To examine the expression of CD200 and its receptor (CD200R) in human chorionic villi during the first trimester of normal pregnancy and early spontaneous abortion (ESA).</p> </sec> <sec id="aogs12476-sec-0002" sec-type="section"> <title>Design</title> <p>Prospective study.</p> </sec> <sec id="aogs12476-sec-0003" sec-type="section"> <title>Methods</title> <p>Expression of CD200 and CD200R in the chorionic villi was determined using streptavidin‐peroxidase immunohistochemistry, confocal laser scanning microscopy and real‐time polymerase chain reaction.</p> </sec> <sec id="aogs12476-sec-0004" sec-type="section"> <title>Population</title> <p>Thirty‐five women diagnosed with ESA and 30 women experiencing a healthy pregnancy in a medical university hospital in China were enrolled in this study between 2011 and 2013.</p> </sec> <sec id="aogs12476-sec-0005" sec-type="section"> <title>Main outcome measures</title> <p>CD200 and CD200R expression.</p> </sec> <sec id="aogs12476-sec-0006" sec-type="section"> <title>Results</title> <p>The expression of CD200 in syncytiotrophoblast cells was significantly higher during normal pregnancy than in ESA (0.51 ± 0.05 vs. 0.35 ± 0.05). In contrast, expression of CD200 in cytotrophoblast cells and CD200R in stromal cells was significantly lower during normal pregnancy when compared with ESA (CD200: 0.16 ± 0.02 vs. 0.32 ± 0.03; CD200R: 0.19 ± 0.03 vs. 0.22 ± 0.02). In villi, the expression of both CD200 protein and CD200R transcripts were significantly higher in healthy first‐trimester pregnancy than in ESA (CD200: 156.89 ± 105.65 vs. 37.51 ± 17.62).</p> </sec> <sec id="aogs12476-sec-0007" sec-type="section"> <title>Conclusions</title> <p>There is an increase in inhibitory properties of human chorionic villi during normal pregnancy. The mechanism underlying ESA might be associated with enhanced expression of CD200 and CD200R in the trophoblast, leading to an upregulation of the immune response during the first trimester of pregnancy.</p> </sec> </abstract>