Functional role of antibodies generated in heifers through immunization with Staphylococcus aureus vaccines in invasion and phagocytosis assays. Issue 1 (26th September 2014)

• Record Type: Journal Article
• Authors: Renna, María S.; Pereyra, Elizabet A.L.; Baravalle, Celina; Camussone, Cecilia M.; Dallard, Bibiana E.; Marcipar, Ivan S.; Calvinho, Luis F.
• Is Part Of: FEMS microbiology letters. Volume 360:Issue 1(2014:Nov.)
• Page Start: 62
• Page End: 69
• Publication Date: 2014-09-26
• Subjects: Microbiology -- Periodicals; 579
• DOI: 10.1111/1574-6968.12588
• Languages: English
• ISSNs: 0378-1097

Abstract

<abstract abstract-type="main" id="fml12588-abs-0001"> <title>Abstract</title> <p>A successful <italic>Staphylococcus aureus</italic> vaccine should elicit a long‐term antibody response that prevents establishment of the infection. The aim of the present study was to evaluate the functional role of antibodies raised against different <italic>S. aureus</italic> CP5 vaccines in invasion to bovine mammary epithelial cells (MAC‐T) and phagocytosis by bovine milk macrophages <italic>in vitro</italic>. Sera and whey from cows immunized with a whole‐cell <italic>S. aureus</italic> CP5 vaccine adjuvanted with Al(OH)<sub>3</sub> or with ISCOM Matrix, significantly reduced internalization of <italic>S. aureus</italic> in MAC‐T cells without significant differences between both groups. The effect of antibodies generated by a <italic>S. aureus</italic> whole‐cell and a lysate vaccine formulated with ISCOM Matrix was also evaluated. Sera and whey from both immunized groups significantly reduced <italic>S. aureus</italic> internalization in MAC‐T cells without significant differences between both groups. Whey antibodies against whole‐cell and lysate vaccines were also able to inhibit internalization in MAC‐T cells of a heterologous <italic>S. aureus</italic> strain. In addition, sera from animals vaccinated with <italic>S. aureus</italic> lysate or bacterin promoted milk macrophage phagocytosis. These results provide an insight into the potential mechanisms by which these vaccines can afford protection to the mammary gland against <italic>S. aureus</italic> intramammary infection.</p> </abstract>