Catechin‐based Procyanidins from Peumus boldus Mol. Aqueous Extract Inhibit Helicobacter pylori Urease and Adherence to Adenocarcinoma Gastric Cells. (23rd May 2014)

• Record Type: Journal Article
• Authors: Pastene, Edgar; Parada, Víctor; Avello, Marcia; Ruiz, Antonieta; García, Apolinaria
• Is Part Of: Phytotherapy research. Volume 28:Number 11(2014:Nov.)
• Page Start: 1637
• Page End: 1645
• Publication Date: 2014-05-23
• Subjects: Materia medica, Vegetable -- Periodicals; Botany, Medical -- Periodicals; Medicinal plants -- Periodicals; Plant Extracts -- therapeutic use -- Periodicals; Plants, Medicinal -- Periodicals; 581.634
• DOI: 10.1002/ptr.5176
• Languages: English
• ISSNs: 0951-418X

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>In this work, the anti‐<italic>Helicobacter pylori</italic> effect of an aqueous extract from dried leaves of <italic>Peumus boldus</italic> Mol. (Monimiaceae) was evaluated. This extract displayed high inhibitory activity against <italic>H</italic>. <italic>pylori</italic> urease. Therefore, in order to clarify the type of substances responsible for such effect, a bioassay‐guided fractionation strategy was carried out. The active compounds in the fractions were characterized through different chromatographic methods (RP‐HPLC; HILIC‐HPLC). The fraction named F5 (mDP = 7.8) from aqueous extract was the most active against <italic>H</italic>. <italic>pylori</italic> urease with an IC<sub>50</sub> = 15.9 µg gallic acid equivalents (GAE)/mL. HPLC analysis evidenced that F5 was composed mainly by catechin‐derived proanthocyanidins (LC‐MS and phloroglucinolysis). The anti‐adherent effect of boldo was assessed by co‐culture of <italic>H</italic>. <italic>pylori</italic> and AGS cells. Both the aqueous extract and F5 showed an anti‐adherent effect in a concentration‐dependent manner. An 89.3% of inhibition was reached at 2.0 mg GAE/mL of boldo extract. In conjunction, our results suggest that boldo extract has a potent anti‐urease activity and anti‐adherent effect against <italic>H</italic>. <italic>pylori</italic>, properties directly linked with the presence of catechin‐derived proanthocyanidins. Copyright © 2014 John Wiley & Sons, Ltd.</p> </abstract>