Gelidium elegans, an Edible Red Seaweed, and Hesperidin Inhibit Lipid Accumulation and Production of Reactive Oxygen Species and Reactive Nitrogen Species in 3T3‐L1 and RAW264.7 Cells. (15th June 2014)

• Record Type: Journal Article
• Authors: Jeon, Hui‐Jeon; Seo, Min‐Jung; Choi, Hyeon‐Son; Lee, Ok‐Hwan; Lee, Boo‐Yong
• Is Part Of: Phytotherapy research. Volume 28:Number 11(2014:Nov.)
• Page Start: 1701
• Page End: 1709
• Publication Date: 2014-06-15
• Subjects: Materia medica, Vegetable -- Periodicals; Botany, Medical -- Periodicals; Medicinal plants -- Periodicals; Plant Extracts -- therapeutic use -- Periodicals; Plants, Medicinal -- Periodicals; 581.634
• DOI: 10.1002/ptr.5186
• Languages: English
• ISSNs: 0951-418X

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <italic>Gelidium elegans</italic> is an edible red alga native to the intertidal area of northeastern Asia. We investigated the effect of <italic>G</italic>. <italic>elegans</italic> extract and its main flavonoids, rutin and hesperidin, on lipid accumulation and the production of reactive oxygen species (ROS) and reactive nitrogen species (RNS) in 3T3‐L1 and RAW264.7 cells. Our data show that <italic>G</italic>. <italic>elegans</italic> extract decreased lipid accumulation and ROS/RNS production in a dose‐dependent manner. The extract also inhibited the mRNA expression of adipogenic transcription factors, such as peroxisome proliferator‐activated receptor gamma and CCAAT/enhancer‐binding protein alpha, while enhancing the protein expression of the antioxidant enzymes superoxide dismutases 1 and 2, glutathione peroxidase, and glutathione reductase compared with controls. In addition, lipopolysaccharide‐induced nitric oxide production was significantly reduced in <italic>G</italic>. <italic>elegans</italic> extract‐treated RAW264.7 cells. In analysis of the effects of <italic>G</italic>. <italic>elegans</italic> flavonoids on lipid accumulation and ROS/RNS production, only hesperidin showed an inhibitory effect on lipid accumulation and ROS production; rutin did not affect adipogenesis and ROS status. The antiadipogenic effect of hesperidin was evidenced by the downregulation of peroxisome proliferator‐activated receptor gamma, CCAAT/enhancer‐binding protein alpha, and fatty acid binding protein 4 gene expression. Collectively, our data suggest that <italic>G</italic>. <italic>elegans</italic> is a potential food source containing antiobesity and antioxidant constituents. Copyright © 2014 John Wiley & Sons, Ltd.</p> </abstract>