Microwave‐assisted 18O labeling of Fmoc‐protected amino acids. (6th August 2014)

• Record Type: Journal Article
• Authors: Modzel, Maciej; Płóciennik, Halina; Kluczyk, Alicja; Stefanowicz, Piotr; Szewczuk, Zbigniew
• Is Part Of: Journal of peptide science. Volume 20:Number 11(2014:Nov.)
• Page Start: 896
• Page End: 900
• Publication Date: 2014-08-06
• Subjects: Peptides -- Periodicals; Peptides -- Periodicals; 572.65
• DOI: 10.1002/psc.2682
• Languages: English
• ISSNs: 1075-2617

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>Recently, there has been an increased interest in isotopical labeling of peptides. Although there are several techniques allowing for a complete labeling of all carboxyl groups in peptides, regioselective labeling would be beneficial in many situations. Such labeling requires the use of ¹⁸O‐labeled Fmoc amino acids. We have designed a method for such labeling that is an improvement on a technique proposed earlier. The new procedure is suitable for microscale synthesis and could be used in peptide and proteomics laboratories. Although for the majority of tested amino acids our method gives good labeling efficiency, it is time consuming. Therefore, we have decided to use microwave‐assisted procedure. This approach resulted in reduction of reaction time to 15 min and increased reaction efficiency. Copyright © 2014 European Peptide Society and John Wiley &amp; Sons, Ltd.</p> </abstract>