Up‐regulation of miR‐582‐5p regulates cellular proliferation of prostate cancer cells under androgen‐deprived conditions. Issue 16 (29th August 2014)

• Record Type: Journal Article
• Authors: Maeno, Atsushi; Terada, Naoki; Uegaki, Masayuki; Goto, Takayuki; Okada, Yoshiyuki; Kobayashi, Takashi; Kamba, Tomomi; Ogawa, Osamu; Inoue, Takahiro
• Is Part Of: Prostate. Volume 74:Issue 16(2014)
• Page Start: 1604
• Page End: 1612
• Publication Date: 2014-08-29
• Subjects: Prostate -- Diseases -- Periodicals; 616
• DOI: 10.1002/pros.22877
• Languages: English
• ISSNs: 0270-4137

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="pros22877-sec-0001" sec-type="section"> <title>BACKGROUND</title> <p>MicroRNAs are noncoding small RNA that negatively regulate target gene expression by binding to the 3′‐UTR of mRNA. Previous studies have shown that several microRNAs play a pivotal role in prostate cancer by acting as oncogenes or tumor suppressors. This study was aimed at identifying microRNAs that contribute to the progression to castration resistant prostate cancer.</p> </sec> <sec id="pros22877-sec-0002" sec-type="section"> <title>METHODS</title> <p>MicroRNAs expression profiles of a xenograft model and cell lines were examined by microarray analysis and real‐time PCR. Functional analysis of miR‐582‐5p in cellular proliferation was examined by cell counting. Furthermore, in order to investigate a candidate target of miR‐582‐5p, microarray analysis and analysis in silico were utilized.</p> </sec> <sec id="pros22877-sec-0003" sec-type="section"> <title>RESULTS</title> <p>MiR‐582‐5p was identified to be up‐regulated at the castration resistant stage of a xenograft model, KUCaP2 and in castration resistant cell line, AILNCaP#1. Overexpression of miR‐582‐5p increased the number and the percentage of S phase of LNCaP cells under androgen deprived condition. Moreover, suppression of miR‐582‐5p decreased the number and the percentage of S phase of AILNCaP#1 cells. Furthermore, we identified that miR‐582‐5p down‐regulates EFNB2 expression, which is down‐regulated at the castration resistant stage of a xenograft model, KUCaP2 and in castration resistant cell line, AILNCaP#1.</p> </sec> <sec id="pros22877-sec-0004" sec-type="section"> <title>CONCLUSIONS</title> <p>Our results suggest that up‐regulation of miR‐582‐5p contributes to an increase in the proliferation of prostate cancer cells under androgen deprived conditions. <italic>Prostate 74: 1604–1612, 2014</italic>. © 2014 Wiley Periodicals, Inc.</p> </sec> </abstract>