A Rapid Extraction and Analysis Method for the Simultaneous Determination of 26 Bioflavonoids in Portulaca Oleracea L. Issue 6 (29th May 2014)

• Record Type: Journal Article
• Authors: Liang, Xiao; Li, Lingzhi; Tian, Jinlong; Wu, Yingying; Gao, Pinyi; Li, Danqi; Zhang, Qingyi; Song, Shaojiang
• Is Part Of: Phytochemical analysis. Volume 25:Issue 6(2014:Nov.)
• Page Start: 537
• Page End: 543
• Publication Date: 2014-05-29
• Subjects: Plants -- Analysis -- Periodicals; Plants -- chemistry -- Periodicals; 572.2
• DOI: 10.1002/pca.2524
• Languages: English
• ISSNs: 0958-0344

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <sec id="pca2524-sec-0001" sec-type="section"> <title>Introduction</title> <p> <italic>Portulaca oleracea</italic> L. (<italic>P</italic>. <italic>oleracea</italic>, purslane) is an edible plant that is widely distributed around the world, and flavonoids are its main bioactive constituents. Therefore, the detection of flavonoids is very important for a better understanding of its pharmacological actions and to monitor the product quality control of <italic>P</italic>. <italic>oleracea</italic>.</p> </sec> <sec id="pca2524-sec-0002" sec-type="section"> <title>Objective</title> <p>To develop a rapid method to extract and determine 26 bioflavonoids in <italic>P</italic>. <italic>oleracea</italic>, based on microwave extraction (MWE) and triple quadrupole‐linear ion trap mass spectrometry.</p> </sec> <sec id="pca2524-sec-0003" sec-type="section"> <title>Methods</title> <p>The optimal conditions of MWE for the extraction of flavonoids from <italic>P</italic>. <italic>oleracea</italic> involved the use of methanol as the extraction solvent, a microwave power of 300 W, an extraction time of 450 s, and a solvent‐to‐solid ratio of 30 mL/g. The samples were analysed using an ultra‐performance liquid chromatograph coupled with a triple quadrupole‐linear ion trap mass spectrometer (UPLC–MS/MS) system.</p> </sec> <sec id="pca2524-sec-0004" sec-type="section"> <title>Results</title> <p>The calibration curves of all 26 analytes showed good linearity (<italic>r</italic> ≥ 0.999) and the intra‐ and interday precisions and repeatability were all within required limits. The mean recoveries measured at three concentrations were higher than 94.2%, with RSDs lower than 2.94% for the targets.</p> </sec> <sec id="pca2524-sec-0005" sec-type="section"> <title>Conclusion</title> <p>The established MWE/UPLC–MS/MS method is a rapid and effective method for quality evaluation of <italic>P</italic>. <italic>oleracea</italic> from different production regions and different harvest periods. Copyright © 2014 John Wiley & Sons, Ltd.</p> </sec> </abstract>