Homing effect of adipose‐derived stem cells to the injured liver: the shift of stromal cell‐derived factor 1 expressions. (12th August 2014)

• Record Type: Journal Article
• Authors: Saito, Yu; Shimada, Mitsuo; Utsunomiya, Tohru; Ikemoto, Tetsuya; Yamada, Shinichiro; Morine, Yuji; Imura, Satoru; Mori, Hiroki; Arakawa, Yusuke; Kanamoto, Mami; Iwahashi, Shuichi; Takasu, Chie
• Is Part Of: Journal of hepato-biliary-pancreatic sciences. Volume 21:Number 12(2014)
• Page Start: 873
• Page End: 880
• Publication Date: 2014-08-12
• Subjects: Liver -- Diseases -- Periodicals; Biliary tract -- Diseases -- Periodicals; Pancreas -- Diseases -- Periodicals; 617.556
• DOI: 10.1002/jhbp.147
• Languages: English
• ISSNs: 1868-6974

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <sec id="jhbp147-sec-0001" sec-type="section"> <title>Background</title> <p>Whether systemically transplanted human adipose‐derived stem cells (ADSCs) homed to the injured liver in nude mice under stress with subsequent hepatectomy (Hx) and ischemia‐reperfusion (I/R) was investigated in the present study. The types of cells in the liver that were involved in the homing of ADSCs were clarified, with focus on the stromal‐derived factor‐1 (SDF‐1)/C‐X‐C chemokine receptor type 4 (CXCR‐4) axis.</p> </sec> <sec id="jhbp147-sec-0002" sec-type="section"> <title>Methods</title> <p>Adipose‐derived stem cells were transplanted intravenously immediately after 70% Hx and I/R. ADSCs were traced by in vivo imaging for 24 h after transplantation and ADSCs were histologically detected in the liver. SDF‐1 and CXCR‐4 expressions in the liver were evaluated by real time RT‐PCR. The immunohistochemical analysis of SDF‐1 was also performed to identify SDF‐1 expressing cells in the liver.</p> </sec> <sec id="jhbp147-sec-0003" sec-type="section"> <title>Results</title> <p>Adipose‐derived stem cells were found in various organs immediately following transplantation and almost accumulated in remnant liver or spleen at 6 h after transplantation. ADSCs were also histologically revealed in the harvested liver. Hx and I/R injury significantly enhanced SDF‐1 expressions regardless of ADSCs transplantation, and only ADSC transplantation increased CXCR‐4 expressions. The predominant SDF‐1 positive cells in the liver were equally identified in parenchymal and non‐parenchymal cells at 6 h, but shifted to non‐parenchymal cells at 24 h after transplantation.</p> </sec> <sec id="jhbp147-sec-0004" sec-type="section"> <title>Conclusions</title> <p>Systemically transplanted ADSCs homed to the injured liver after transplantation, possibly based on the mechanisms of SDF‐1/CXCR‐4 axis. Therefore, systemic transplantation might be an effective and practical route for the transplantation of ADSCs.</p> </sec> </abstract>