Inflammation‐Induced Downregulation of Butyrate Uptake and Oxidation Is Not Caused by a Reduced Gene Expression. Issue 2 (February 2015)

• Record Type: Journal Article
• Authors: Boesmans, Leen; Ramakers, Meine; Arijs, Ingrid; Windey, Karen; Vanhove, Wiebe; Schuit, Frans; Rutgeerts, Paul; Verbeke, Kristin; De Preter, Vicky
• Is Part Of: Journal of cellular physiology. Volume 230:Issue 2(2015:Feb.)
• Page Start: 418
• Page End: 426
• Publication Date: 2015-02
• Subjects: Physiology -- Periodicals; Cell physiology -- Periodicals; 571.6
• DOI: 10.1002/jcp.24725
• Languages: English
• ISSNs: 0021-9541

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <sec id="jcp24725-sec-0001" sec-type="section"> <p>In ulcerative colitis (UC) the butyrate metabolism is impaired, leading to energy‐deficiency in the colonic cells. The effect of inflammation on the butyrate metabolism was investigated. HT‐29 cells were incubated with pro‐inflammatory cytokines (TNF‐α and/or IFN‐γ) for 1 and 24 h. Cells were additionally stimulated with butyrate to investigate its anti‐inflammatory potential. Butyrate uptake and oxidation were measured using <sup>14</sup>C‐labeled butyrate. Gene expression of the butyrate metabolism enzymes, interleukin 8 (<italic>IL‐8</italic>; inflammatory marker) and villin‐1 (<italic>VIL‐1</italic>; epithelial cell damage marker) was measured via quantitative RT‐PCR. Significantly increased <italic>IL‐8</italic> expression and decreased <italic>VIL‐1</italic> expression after 24 h incubation with TNF‐α and/or IFN‐γ confirmed the presence of inflammation. These conditions induced a decrease of both butyrate uptake and oxidation, whereas the gene expression was not reduced. Simultaneous incubation with butyrate counteracted the reduced butyrate oxidation. In contrast, 1 h incubation with TNF‐α induced a significant increased <italic>IL‐8</italic> expression and decreased butyrate uptake. Incubation with TNF‐α and/or IFN‐γ for 1 h did not induce cell damage nor influence butyrate oxidation. The inflammation‐induced downregulation of the butyrate metabolism was not caused by a reduced gene expression, but appeared consequential to a decreased butyrate uptake. Increasing the luminal butyrate levels might have therapeutic potential in UC. J. Cell. Physiol. 230: 418–426, 2015. © 2014 Wiley Periodicals, Inc.</p> </sec> </abstract>