Temporal characterization of lymphatic metastasis in an orthotopic mouse model of oral cancer. Issue 11 (27th January 2014)

• Record Type: Journal Article
• Authors: Szaniszlo, Peter; Fennewald, Susan M.; Qiu, Suimin; Kantara, Carla; Shilagard, Tuya; Vargas, Gracie; Resto, Vicente A.
• Is Part Of: Head & neck. Volume 36:Issue 11(2014:Nov.)
• Page Start: 1638
• Page End: 1647
• Publication Date: 2014-01-27
• Subjects: Head -- Diseases -- Periodicals; Neck -- Diseases -- Periodicals; Head -- Periodicals; Neck -- Periodicals; Face -- Periodicals; 617.51059
• DOI: 10.1002/hed.23500
• Languages: English
• ISSNs: 1043-3074

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <sec id="hed23500-sec-0001" sec-type="section"> <title>Background</title> <p>The overall mortality rate in cases of head and neck squamous cell carcinoma (HNSCC) has not improved over the past 30 years, mostly because of the high treatment failure rate among patients with regionally metastatic disease. To better understand the pathobiologic processes leading to lymphatic metastasis development, there is an urgent need for relevant animal models.</p> </sec> <sec id="hed23500-sec-0002" sec-type="section"> <title>Methods</title> <p>HNSCC cell lines were implanted into the tongues of athymic nude mice. Histology, immunohistochemistry, and ex vivo 2‐photon microscopy were used to evaluate tumor progress and spread.</p> </sec> <sec id="hed23500-sec-0003" sec-type="section"> <title>Results</title> <p>Orthotopic xenografts of different HNSCC cell lines produced distinct patterns of survival, tumor histology, disease progression rate, and lymph node metastasis development. Remarkably, all injected cell types reached the lymph nodes within 24 hours after injection, but not all developed metastasis.</p> </sec> <sec id="hed23500-sec-0004" sec-type="section"> <title>Conclusion</title> <p>This orthotopic xenograft model closely mimics several characteristics of human cancer and could be extremely valuable for translational studies focusing on lymphatic metastasis development and pathobiology. © 2014 Wiley Periodicals, Inc. <italic>Head Neck</italic> 36: 1638–1647, 2014</p> </sec> </abstract>