Direct Determination of miR‐21 in Total RNA Extracted from Breast Cancer Samples Using Magnetosensing Platforms and the p19 Viral Protein as Detector Bioreceptor. Issue 10 (10th September 2014)

• Record Type: Journal Article
• Authors: Torrente‐Rodríguez, R. M.; Campuzano, S.; López‐Hernández, E.; Granados, R.; Sánchez‐Puelles, J. M.; Pingarrón, J. M.
• Is Part Of: Electroanalysis. Volume 26:Issue 10(2014:Oct.)
• Page Start: 2080
• Page End: 2087
• Publication Date: 2014-09-10
• Subjects: Electrochemical analysis -- Periodicals; Chemistry, Analytical -- Periodicals; Electrochemistry -- Periodicals; 543.4
• DOI: 10.1002/elan.201400317
• Languages: English
• ISSNs: 1040-0397

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>A novel magnetobiosensing approach for the rapid, sensitive and selective miR‐21 detection is reported involving the use of a specific RNA probe (antimiR‐21), streptavidin‐magnetic beads (Strep‐MBs), the siRNA Binding Protein p19 as detector bioreceptor, and amperometric detection with the H₂O₂/hydroquinone (HQ) system at disposable screen‐printed carbon electrodes. The magnetosensor exhibited a dynamic range from 1.4 to 10 nM and a detection limit of 4.2 fmol of synthetic target miR‐21 without any amplification step in 75 min. The usefulness of the approach was evaluated by analyzing total RNA (RNA_t) extracted from metastatic breast cancer cell lines, human tissues and breast cytologies.</p> </abstract>