Dynamic Structures of DNA Heptamers with Different Central Trinucleotide Sequences Studied by Electrochemical and Spectral Methods. Issue 10 (15th September 2014)

• Record Type: Journal Article
• Authors: Pilarova, Iveta; Kejnovska, Iva; Vorlickova, Michaela; Trnkova, Libuse
• Is Part Of: Electroanalysis. Volume 26:Issue 10(2014:Oct.)
• Page Start: 2118
• Page End: 2128
• Publication Date: 2014-09-15
• Subjects: Electrochemical analysis -- Periodicals; Chemistry, Analytical -- Periodicals; Electrochemistry -- Periodicals; 543.4
• DOI: 10.1002/elan.201400288
• Languages: English
• ISSNs: 1040-0397

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>A short, single‐stranded DNA fragment d(GCGAAGC) and its analogs d(GC<italic>NNN</italic>GC) carrying different trinucleotide sequences in the center of the molecule (AAA, CCC, GGG or TTT) were investigated by spectral (CD and UV spectra) and electrochemical methods (voltammetry, polyacrylamide gel electrophoresis). Our results show that (i) the central triplet GAA or AAA dramatically stabilizes DNA hairpins, (ii) a stem‐loop configuration is not supported by CCC or TTT sequences, instead, these heptamers adopt bimolecular duplex forms. Finally (iii), in the case of GGG sequence a very stable supramolecular G‐quadruplex is formed.</p> </abstract>