Flow cytometric assessment of morphology, viability, and production of reactive oxygen species of Crassostrea gigas oocytes. Application to Toxic dinoflagellate (Alexandrium minutum) exposure. Issue 12 (18th October 2014)

• Record Type: Journal Article
• Authors: Le Goïc, Nelly; Hégaret, Hélène; Boulais, Myrina; Béguel, Jean‐Philippe; Lambert, Christophe; Fabioux, Caroline; Soudant, Philippe
• Is Part Of: Cytometry. Volume 85:Issue 12(2014:Dec.)
• Page Start: 1049
• Page End: 1056
• Publication Date: 2014-10-18
• Subjects: Flow cytometry -- Periodicals; Imaging systems in biology -- Periodicals; Imaging systems in medicine -- Periodicals; Diagnostic imaging -- Periodicals; 571.605
• DOI: 10.1002/cyto.a.22577
• Languages: English
• ISSNs: 1552-4922

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <p>The Pacific oyster <italic>Crassostrea gigas</italic> accounts for a large part of shellfish aquaculture production worldwide. Aspects of morphological and functional characteristics of oyster oocytes remain poorly documented, and traditional techniques, such as microscopic observations of shape or fertilization rate, are time and space consuming. The purpose of this study was to assess for the first time viability and reactive oxygen species (ROS) production of Pacific oyster oocytes using flow cytometry (FCM) and to apply this method to determine oocyte responses to in vitro exposure to the toxic dinoflagellate <italic>Alexandrium minutum</italic>. A culture of <italic>A. minutum</italic> caused a significant increase in oocyte ROS production, which gradually increased with the age of the culture, but viability was not affected. Effect of the supernatant of the same <italic>A. minutum</italic> culture did not cause any significant modifications of oocyte morphology, viability, or ROS level. This study confirmed that some oocyte cellular characteristics can be assessed using FCM techniques. © 2014 International Society for Advancement of Cytometry</p> </abstract>