Cometabolic Degradation Kinetics of Trichloroethylene Based on Toluene Enhancement by Encapsulated Burkholderia cepacia G4. Issue 11 (16th April 2014)

• Record Type: Journal Article
• Authors: Hamid, Shanawar; Amin, Muhammad T.; Alazba, Abdulrahman A.; Manzoor, Umair; Amin, Muhammad N.
• Is Part Of: Clean. Volume 42:Issue 11(2014:Nov.)
• Page Start: 1642
• Page End: 1649
• Publication Date: 2014-04-16
• Subjects: Water quality -- Periodicals; Water -- Pollution -- Periodicals; Pollution -- Periodicals; Bioremediation -- Periodicals; Sewage -- Periodicals; Water chemistry -- Periodicals; 333.7205
• DOI: 10.1002/clen.201300725
• Languages: English
• ISSNs: 1863-0650

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <sec id="clen201300725-sec-0001" sec-type="section"> <p>The ability of encapsulated <italic>Burkholderia cepacia</italic> G4 (ATCC 53617) for trichloroethylene (TCE) degradation (1.5, 5, 10, and 20 mg/L) in the presence of toluene (10 and 60 mg/L) as enhancement substrate was evaluated experimentally. <italic>Burkholderia</italic><italic>cepacia</italic> G4 cultures were encapsulated in cylindrical pellets (4 mm in diameter and 4 mm in height (preferred)) using polyethylene glycol (PEG). Higher transformation capacities were observed for the encapsulated cultures for both toluene concentrations. The highest transformation capacities measured for the encapsulated cultures and suspended cultures were 46.98 and 5.94 μg TCE/mg biomass, respectively. The Monod equation was used to simulate the degradation rates of toluene and Haldane's equation was employed to describe the degradation kinetics of TCE. The first‐order reaction rate constant (<italic>k</italic>/<italic>K</italic><sub>s</sub>) for toluene degradation in the encapsulated cultures was 2.3‐fold higher than the value of the suspended cultures, whereas the <italic>k</italic><sub>c</sub>/<italic>K</italic><sub>sc</sub> value for TCE was 4.3‐fold higher compared to the suspended cultures. The higher kinetic values of the encapsulated cultures indicate that the degradation efficiency and capability of <italic>B. cepacia</italic> G4 was enhanced through PEG encapsulation. Moreover, the higher inhibition constant value for the encapsulated cultures compared with the suspended cultures demonstrated that PEG‐encapsulated <italic>B. cepacia</italic> G4 can tolerate and degrade much higher TCE concentrations.</p> </sec> </abstract>