Peptide and peptide nucleic acid syntheses using a DNA/RNA synthesizer. Issue 6 (November 2014)

• Record Type: Journal Article
• Authors: Pokharel, Durga; Fueangfung, Suntara; Zhang, Mingcui; Fang, Shiyue
• Is Part Of: Biopolymers. Volume 102:Issue 6(2014)
• Page Start: 487
• Page End: 493
• Publication Date: 2014-11
• Subjects: Biopolymers -- Periodicals; Peptides -- Periodicals; Spectrum analysis -- Periodicals; 572.33
• DOI: 10.1002/bip.22574
• Languages: English
• ISSNs: 0006-3525

Abstract

<abstract abstract-type="main"> <title>ABSTRACT</title> <p>The use of an ABI 394 DNA/RNA synthesizer for peptide and peptide nucleic acid (PNA) syntheses is described. No additional physical part or software is needed for the application. A commercially available large DNA synthesis column was used, and only about half of its volume was filled with resin when the resin was fully swollen. With additional space in the top portion of the column, agitation of reaction mixture was achieved by bubbling argon from the bottom without losing solution. Removing solutions from column was achieved by flushing argon from top to bottom. Two peptide and two PNA sequences were synthesized. Good yields were obtained in all the cases. The method is easy to follow by researchers who are familiar with DNA/RNA synthesizer. © 2014 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 102: 487–493, 2014.</p> </abstract>