Application of a C6‐OH of chitosan immobilized cyclodextrin derivates on an electrochemical H2O2 biosensor. Issue 8 (7th October 2014)

• Record Type: Journal Article
• Authors: Yu, Chen; Weiping, Li; Yanchun, Ye; JianQiang, Geng; Yanwen, Guo; Tianwei, Wang; Liye, Wang; Huimin, Tan
• Is Part Of: Journal of applied polymer science. Volume 132:Issue 8(2015:Apr. 15)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2014-10-07
• Subjects: Polymers -- Periodicals; Polymerization -- Periodicals; 668.9
• DOI: 10.1002/app.41499
• Languages: English
• ISSNs: 0021-8995

Abstract

<abstract abstract-type="main"> <title>ABSTRACT</title> <p>Biosensor detecting techniques have attracted much attention in the content determination of H<sub>2</sub>O<sub>2</sub>, which has been used illegally as a food additive. An electrochemical biosensing membrane for the detection of H<sub>2</sub>O<sub>2</sub> was developed with C6‐OH of chitosan immobilized cyclodextrin derivates (6‐CD–CTS), which possessed a high cyclodextrin loading capacity (2.12 × 10<sup>−4</sup> mol/g), as the carrier. The biosensor was prepared through the inclusion of ferrocene as the electron mediator in a hydrophobic cavity of cyclodextrin and crosslinking catalase (CAT) to 2‐NH<sub>2</sub> of 6‐CD–CTS. The ferrocene‐included complex was evaluated by ultraviolet–visible spectrophotometry and thermogravimetric analysis. Its electrochemical behavior was also studied. The impact of the reaction conditions on the CAT immobilization capacity was evaluated. When previous membrane was used to detect the concentration of H<sub>2</sub>O<sub>2</sub> (<italic>C</italic><sub>H2O2</sub>), we found that the catalysis of CAT and the signal amplification of ferrocene had a major impact on the cyclic voltammograms. The optimal working pH of the modified electrode was 7.0. The peak current (<italic>I</italic>) had a linear relationship with the H<sub>2</sub>O<sub>2</sub> concentration (CH<sub>2</sub>O<sub>2</sub>) in the range 1.0 × 10<sup>−4</sup> to 1.0 × 10<sup>−3</sup> mol/L. The linear regression equation was <italic>I</italic> = 0.00475<italic>C</italic><sub>H2O2</sub> − 0.03025. The detection limit was 10<sup>−6</sup> mol/L. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. <bold>2015</bold>, <italic>132</italic>, 41499.</p> </abstract>