Transabdominal coelocentesis as early source of fetal DNA for chromosomal and molecular diagnosis. (November 2014)

• Record Type: Journal Article
• Authors: Pietropolli, Adalgisa; Vicario, Roberta; Peconi, Cristina; Zampatti, Stefania; Quitadamo, Maria Chiara; Capogna, Maria Vittoria; Ragazzo, Michele; Nardone, Anna Maria; Postorivo, Diana; Spitalieri, Paola; Sarta, Simona; Ratto, Fabrizio; Novelli, Giuseppe; Sangiuolo, Federica; Piccione, Emilio; Giardina, Emiliano
• Is Part Of: Journal of maternal-fetal & neonatal medicine. Volume 27:Number 16(2014)
• Page Start: 1656
• Page End: 1660
• Publication Date: 2014-11
• Subjects: Obstetrics -- Periodicals; Perinatology -- Periodicals; Infants (Newborn) -- Diseases -- Periodicals; Neonatology -- Periodicals; 618.2
• DOI: 10.3109/14767058.2013.871697
• Languages: English
• ISSNs: 1476-7058

Abstract

<abstract> <title>Abstract</title> <p>This study reports a comparative analysis between results of transabdominal coelocentesis and traditional invasive procedure in order to assess the usefulness of coelocentesis as a source of fetal DNA for molecular and chromosomal analysis. A number of 28 women were included in the study. A successful sampling of coelomic fluid was obtained in 25 women by transabdominal procedure. A positive amplification of DNA with QF-PCR techniques was obtained in 90% of cases, while 10% of cases failed to reveal interpretable results. Although all samples were cultured, the growth rate was not sufficient to determine karyotypes within 2 weeks. Five samples were selected to be analyzed by array-based comparative genomic hybridization (a-CGH) but the interpretation of these results was difficult and ambiguous. Our results suggest that transabdominal coelocentesis is suitable for the detection of single DNA variation and for QF-PCR analysis, while further experiments are needed to develop optimized protocols for traditional karyotyping and array-analysis.</p> </abstract>