In vivo anti-inflammatory and analgesic activities of strictosamide from Nauclea officinalis. (November 2014)

• Record Type: Journal Article
• Authors: Li, Na; Cao, Liang; Cheng, Yue; Meng, Zhao-Qing; Tang, Zhao-Hui; Liu, Wen-Jun; Wang, Zhen-Zhong; Ding, Gang; Xiao, Wei
• Is Part Of: Pharmaceutical biology. Volume 52:Number 11(2014:Nov.)
• Page Start: 1445
• Page End: 1450
• Publication Date: 2014-11
• Subjects: Pharmacognosy -- Periodicals; Materia medica, Vegetable -- Periodicals; 615.321
• DOI: 10.3109/13880209.2014.895910
• Languages: English
• ISSNs: 1388-0209

Abstract

<abstract> <title>Abstract</title> <p> <italic>Context</italic>: Strictosamide is the main representative constituent of <italic>Nauclea officinalis</italic> Pierre ex Pitard (Rubiaceae), which has been used for a long time in China to treat diseases related to infection and inflammation, but its pharmacological activities are not well studied.</p> <p> <italic>Objective</italic>: This work evaluates the anti-inflammatory and analgesic activities of strictosamide by <italic>in vivo</italic> experiments.</p> <p> <italic>Materials and methods</italic>: The anti-inflammatory activity was assessed in mice by models of 12-<italic>O</italic>-tetradecanoylphorbol-13-acetate (TPA)-induced ear edema, acetic acid-elevated vascular permeability, and carboxymethylcellulose sodium (CMC–Na)-induced leukocyte migration. The analgesic activity was estimated in mice using acetic acid-induced writhing and hot-plate tests. Compound was injected to mice twice a day for 3 d at doses of 10, 20, and 40 mg/kg.</p> <p> <italic>Results</italic>: At 20 and 40 mg/kg, strictosamide obviously decreased the TPA-induced mice ear edema (24.7 and 28.1% inhibition, respectively), and significantly inhibited acetic acid-stimulated peritoneal vascular permeability in mice (23.3 and 33.4% inhibition, respectively). It also significantly decreased the leukocytes in the mice peritoneal cavity induced by CMC–Na at all the tested doses (46.0, 49.1, and 58.7% inhibition, respectively). To acetic acid-induced writhing test in mice, strictosamide markedly prolonged the pain latency at 20 and 40 mg/kg and decreased the writhing counts at 40 mg/kg (49.7% inhibition). However, it did not obviously improve the pain threshold of mice in hot-plate test.</p> <p> <italic>Discussion and conclusion</italic>: Strictosamide may have important effects on inflammation and inflammatory pain. The results provide scientific support for the role of strictosamide in the use of <italic>N. officinalis</italic> to treat inflammatory diseases.</p> </abstract>