Lipidomics investigation of reversal effect of glycyrrhizin (GL) towards lithocholic acid (LCA)-induced alteration of phospholipid profiles. (December 2014)

• Record Type: Journal Article
• Authors: Han, Jing-Chun; Yu, Jian; Gao, Ya-Jie
• Is Part Of: Pharmaceutical biology. Volume 52:Number 12(2014:Dec.)
• Page Start: 1624
• Page End: 1628
• Publication Date: 2014-12
• Subjects: Pharmacognosy -- Periodicals; Materia medica, Vegetable -- Periodicals; 615.321
• DOI: 10.3109/13880209.2014.900810
• Languages: English
• ISSNs: 1388-0209

Abstract

<abstract> <title>Abstract</title> <p> <italic>Context</italic>: Glycyrrhizin (GL), the major ingredient isolated from licorice, exerts multiple pharmacological activities.</p> <p> <italic>Objective</italic>: To elucidate the protective mechanism of GL towards lithocholic acid (LCA)-induced liver toxicity using lipidomics.</p> <p> <italic>Materials and methods</italic>: GL (200 mg/kg) dissolved in corn oil was treated intraperitoneally for 7 d. On the 4th day, 200 mg/kg LCA was used to treat mice (i.p., twice daily) for another 4 d. The protective role of GL towards LCA-induced liver toxicity was investigated through evaluating the liver histology and the activity of alanine transaminase (ALT). The complete lipid profile was employed using UFLC-Triple TOF MS-based lipidomics.</p> <p> <italic>Results</italic>: Intraperitoneal (i.p.) administration of 200 mg/kg GL can significantly protect LCA-induced liver damage, indicated by alleviated histology alteration and prevention of the ALT elevation. Lipidomics analysis can well separate the control group from LCA-treated group, and three lipid components were major contributors, including LPC 16:0, LPC 18:0, and LPC 18:2. GL treatment can significantly prevent LCA-induced reduction of these three lipid compounds, providing a new explanation for GL's protection mechanism towards LCA-induced liver toxicity.</p> <p> <italic>Discussion and conclusion</italic>: The recent study highlights the importance of lipidomics in elucidating the therapeutic mechanism of herbs.</p> </abstract>