AAPE proliposomes for topical atopic dermatitis treatment. (December 2014)

• Record Type: Journal Article
• Authors: Jahn, Alexander; Song, Chung Kil; Balakrishnan, Prabagar; Hong, Soon-Sun; Lee, Ju-Hee; Chung, Suk-Jae; Kim, Dae-Duk
• Is Part Of: Journal of microencapsulation. Volume 31:Number 8(2014:Dec.)
• Page Start: 768
• Page End: 773
• Publication Date: 2014-12
• Subjects: Microencapsulation -- Periodicals; Controlled release technology -- Periodicals; 615.19
• DOI: 10.3109/02652048.2014.932027
• Languages: English
• ISSNs: 0265-2048

Abstract

<abstract> <title>Abstract</title> <p> <italic>Context</italic>: Anti-inflammatory effect of advanced adipose stem cell derived protein extract (AAPE) could be improved by minimising protein degradation. <italic>Objective</italic>: To develop a proliposomal formulation of AAPE for the treatment of topical atopic dermatitis. <italic>Materials and methods</italic>: Proliposomal powder was manufactured by evaporating a solution of soy phosphatidyl choline, AAPE and Poloxamer 407 in ethanol under vacuum on sorbitol powder. Characterisation of proliposomes (zeta potential, diameter, stability and flowability) as well as <italic>in vivo</italic> efficacy in a dermatitis mouse model was investigated. <italic>Results and discussion</italic>: Reconstitution of the proliposomal powder formed liposomes of 589 ± 3.6 nm diameter with zeta potential of −51.33 ± 0.36 mV. Protein stability was maintained up to 90 days at 25 °C as proliposomes. <italic>In vivo</italic> studies on atopic dermatitis mouse model showed a significant reduction in IgE levels after topical AAPE proliposome treatment. <italic>Conclusion</italic>: AAPE proliposomes maintained protein stability and showed promising results for atopic dermatitis treatment.</p> </abstract>