Crystallization and preliminary X‐ray analysis of the hypothetical deaminase RPB_0146 from Rhodopseudomonas palustris HaA2. Issue 11 (5th November 2014) - Zhang, Guofang
Abstract RPB_0146, a putative deaminase from Rhodopseudomonas palustris HaA2, was expressed in Escherichia coli BL21 (DE3) cells and purified using a His6 tag by Ni2+‐chelating affinity chromatography for X‐ray crystallographic analysis. Diffraction‐quality crystals were grown by the hanging‐drop vapour‐diffusion method at 289 K and diffracted to a resolution of 2.44 Å using a wavelength of 1.000 Å at the Photon Factory (KEK), Japan. The crystals belonged to the orthorhombic space group P212121, with unit‐cell parameters a = 66.26, b = 123.94, c = 155.95 Å.