Chemical composition and evaluation of modulatory of the antibiotic activity from extract and essential oil of Myracrodruon urundeuva. (May 2014)

• Record Type: Journal Article
• Authors: Figueredo, Fernando G.; Lucena, Bruno F. F.; Tintino, Saulo R.; Matias, Edinardo F. F.; Leite, Nadghia F.; Andrade, Jacqueline C.; Nogueira, Lavouisier F. B.; Morais, Edson C.; Costa, José G. M.; Coutinho, Henrique D. M.; Rodrigues, Fabiola F. G.
• Is Part Of: Pharmaceutical biology. Volume 52:Number 5(2014:May)
• Page Start: 560
• Page End: 565
• Publication Date: 2014-05
• Subjects: Pharmacognosy -- Periodicals; Materia medica, Vegetable -- Periodicals; 615.321
• DOI: 10.3109/13880209.2013.853810
• Languages: English
• ISSNs: 1388-0209

Abstract

<abstract> <title>Abstract</title> <p> <italic>Context</italic>: The combination of antibiotics with natural products has demonstrated promising synergistic effects in several therapeutic studies.</p> <p> <italic>Objective</italic>: The aim of this study was to determine the effect of a combination of an ethanol extract of <italic>Myracrodruon urundeuva</italic> Fr. All. (Anacardiaceae) (aroeira plant) and its essential oil with six antimicrobial drugs against multiresistant strains of <italic>Staphylococcus aureus</italic> and <italic>Escherichia coli</italic> from clinical isolates.</p> <p> <italic>Materials and methods</italic>: After identification of the chemical components by GC-MS, the antibacterial activity of the natural products and antibiotics was assessed by determining the minimal inhibitory concentration (MIC) using the microdilution method and concentrations ranging 8–512 μg/mL and 0.0012–2.5 mg/mL, respectively. Assays were performed to test for a possible synergistic action between the plant products and the antimicrobials, using the extract and the oil at a sub-inhibitory concentration (128 μg/mL) and antibiotic at concentrations varying between 8 and 512 μg/mL.</p> <p> <italic>Results</italic>: The GC-MS analysis identified the main compound as δ-carene (80.41%). The MIC of the natural products was >1024 μg/mL, except against <italic>S. aureus</italic> ATCC25923. Only the combinations of the natural products with gentamicin, amikacin and clindamycin were effective against <italic>S. aureus</italic> 358, enhancing the antibiotic activity by reducing the MIC.</p> <p> <italic>Conclusions</italic>: The extract from aroeira showed a higher antibacterial activity and the oil was more effective in potentiating the activity of conventional antibiotics.</p> </abstract>