Ginsenoside Rh1 induces mouse osteoblast growth and differentiation through the bone morphogenetic protein 2/runt‐related gene 2 signalling pathway. (24th August 2014)

• Record Type: Journal Article
• Authors: Siddiqi, Muhammad Hanif; Siddiqi, Muhammad Zubair; Ahn, Sungeun; Kim, Yeon‐Ju; Yang, Deok Chun
• Is Part Of: Journal of pharmacy and pharmacology. Volume 66:Number 12(2014:Dec.)
• Page Start: 1763
• Page End: 1773
• Publication Date: 2014-08-24
• Subjects: Pharmacy -- Periodicals; Pharmacology -- Periodicals; 615.1
• DOI: 10.1111/jphp.12306
• Languages: English
• ISSNs: 0022-3573

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <sec id="jphp12306-sec-0001" sec-type="section"> <title>Objectives</title> <p>This study aimed to investigate the stimulative and pharmacological effects of ginsenoside Rh1 (hereinafter referred to as: Rh1) on differentiation and mineralization of osteoblast and its possible mechanism of action on the expression of bone morphogenetic protein 2 (BMP‐2)/Runt‐related gene 2 (Runx2) signalling pathways using mouse preosteoblastic MC3T3‐E1 cell line as in‐vitro model.</p> </sec> <sec id="jphp12306-sec-0002" sec-type="section"> <title>Methods</title> <p>An in‐vitro stimulative activity of Rh1 was assessed by analyzing alkaline phosphatase activity (ALP), type‐I collagen (Coll‐I) synthesis, mineralization and glutathione content. Its antioxidant activity was measured by evaluating the reactive oxygen species (ROS) production in the presence of antimycin A (AMA), one of the mitochondrial dysfunction factors. The level of BMP‐2/Runx2 signal‐regulated osteoblast‐specific proteins such as osteocalcin (OCN), Coll‐I and ALP were detected using Western blot analysis.</p> </sec> <sec id="jphp12306-sec-0003" sec-type="section"> <title>Key findings</title> <p>Rh1 was capable to stimulate cell growth, ALP activity, Coll‐I synthesis, mineralization and glutathione content in the MC3T3‐E1 cells. BMP‐2 and Runx2 expression were also increased by Rh1 concentration dependently. Additionally, Rh1 also showed inhibitory action on the level of ROS production enhanced by AMA in MC3T3‐E1 cells. Rh1 could increase the expression level of BMP‐2/Runx2 signal‐regulated osteogenic markers such as ALP, Coll‐I and OCN.</p> </sec> <sec id="jphp12306-sec-0004" sec-type="section"> <title>Conclusions</title> <p>Rh1, a protopanaxatriol type's active ingredients of <italic>P</italic><italic>anax ginseng</italic> Meyer, possesses osteoblast differentiation, osteogenic stimulatory and anti‐oxidative activity.</p> </sec> </abstract>