Total polysaccharide of Yupingfeng protects against bleomycin‐induced pulmonary fibrosis via inhibiting transforming growth factor‐β1‐mediated type I collagen abnormal deposition in rats. (11th September 2014)

• Record Type: Journal Article
• Authors: Xu, Liang; Li, Liu‐cheng; Zhao, Ping; Qi, Lian‐wen; Li, Ping; Gao, Jian; Fei, Guang‐he
• Is Part Of: Journal of pharmacy and pharmacology. Volume 66:Number 12(2014:Dec.)
• Page Start: 1786
• Page End: 1795
• Publication Date: 2014-09-11
• Subjects: Pharmacy -- Periodicals; Pharmacology -- Periodicals; 615.1
• DOI: 10.1111/jphp.12308
• Languages: English
• ISSNs: 0022-3573

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <sec id="jphp12308-sec-0001" sec-type="section"> <title>Objective</title> <p>This study was to explore the antifibrotic effect and the possible mechanism of total polysaccharides of <italic>Y</italic><italic>upingfeng</italic> (YPF‐P) on bleomycin (BLM)‐induced pulmonary fibrosis in rats.</p> </sec> <sec id="jphp12308-sec-0002" sec-type="section"> <title>Methods</title> <p>Pulmonary fibrosis was induced in Sprague‐Dawley rats by BLM (5 mg/kg), killed 14 and 28 days after BLM administration by abdominal aorta exsanguination and removed the lungs. Lung coefficient was counted at the same time. Besides, H&E and Masson's trichrome staining for histopathological changes of lung tissues were observed. Additionally, western blotting and immunohistochemical staining techniques were used to detect expression of transforming growth factor‐β1 (TGF‐β1), type I collagen (Col‐I) and α‐smooth muscle actin (α‐SMA). Finally, the levels of Col‐I and hydroxyproline (HYP) in lung tissues were also utilized.</p> </sec> <sec id="jphp12308-sec-0003" sec-type="section"> <title>Key findings</title> <p>YPF‐P alleviated the increase of lung coefficient induced by BLM instillation in pulmonary fibrosis rat, pathologic changes and collagen distribution were obviously ameliorated, while the increase of α‐SMA‐positive cells and TGF‐β1 expression was prevented after YPF‐P treatment. Moreover, the contents of HYP and Col‐I were decreased in YPF‐P group.</p> </sec> <sec id="jphp12308-sec-0004" sec-type="section"> <title>Conclusions</title> <p>YPF‐P had antifibrotic effect in experiment, which may reduce the synthesis and promote the deposition of Col‐I via suppressing the increase of TGF‐β1‐mediated activation of myofibroblasts.</p> </sec> </abstract>