Phototrophic growth of Arthrospira platensis in a respiration activity monitoring system for shake flasks (RAMOS®). Issue 6 (24th June 2014)

• Record Type: Journal Article
• Authors: Socher, Maria Lisa; Lenk, Felix; Geipel, Katja; Schott, Carolin; Püschel, Joachim; Haas, Christiane; Grasse, Christiane; Bley, Thomas; Steingroewer, Juliane
• Is Part Of: Engineering in life sciences. Volume 14:Issue 6(2014:Nov.)
• Page Start: 658
• Page End: 666
• Publication Date: 2014-06-24
• Subjects: Bioengineering -- Periodicals; 660.605
• DOI: 10.1002/elsc.201300156
• Languages: English
• ISSNs: 1618-0240

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>Optimizing illumination is essential for optimizing the growth of phototrophic cells and their production of desired metabolites and/or biomass. This requires appropriate modulation of light and other key inputs and continuous online monitoring of their metabolic activities. Powerful noninvasive systems for cultivating heterotrophic organisms include shake flasks in online monitoring units, but they are rarely used for phototrophs because they lack the appropriate illumination design and necessary illuminatory power. This study presents the design and characterization of a photosynthetic shake flask unit, illuminated from below by warm white light‐emitting diodes with variable light intensities up to 2300 μmol m<sup>−2</sup> s<sup>−1</sup>. The photosynthetic unit was successfully used, in combination with online monitoring of oxygen production, to cultivate <italic>Arthrospira platensis</italic>. In phototrophic growth under continuous light and a 16 h light/8 h dark cycle (light intensity: 180 μmol m<sup>−2</sup> s<sup>−1</sup>), the oxygen transfer rate and biomass‐related oxygen production were −1.5 mmol L<sup>−1</sup> h<sup>−1</sup> and 0.18 mmol O<sub>2</sub> g<sub>x</sub><sup>−1</sup> h<sup>−1</sup>, respectively. The maximum specific growth rate was 0.058 h<sup>−1</sup>, during the exponential growth phase, after which the biomass concentration reached 0.75 g L<sup>−1</sup>.</p> </abstract>