Production, crystallization and preliminary crystallographic analysis of Allochromatium vinosum thiosulfate dehydrogenase TsdA, an unusual acidophilic c‐type cytochrome. Issue 10 (1st October 2014)

• Record Type: Journal Article
• Authors: Brito, José A.; Gutierres, André; Denkmann, Kevin; Dahl, Christiane; Archer, Margarida
• Is Part Of: Acta crystallographica. Volume 70:Issue 10(2014:Oct.)
• Page Start: 1424
• Page End: 1427
• Publication Date: 2014-10-01
• Subjects: Crystallography -- Periodicals; Crystals -- Periodicals; 548
• DOI: 10.1107/S2053230X14019384
• Languages: English
• ISSNs: 2053-230X

Abstract

<abstract abstract-type="main" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p>The ability to perform the very simple oxidation of two molecules of thiosulfate to tetrathionate is widespread among prokaryotes. Despite the prevalent occurrence of tetrathionate formation and its well documented significance within the sulfur cycle, little is known about the enzymes that catalyze the oxidative condensation of two thiosulfate anions. To fill this gap, the thiosulfate dehydrogenase (TsdA) enzyme from the purple sulfur bacterium <italic>Allochromatium vinosum</italic> was recombinantly expressed in <italic>Escherichia coli</italic>, purified and crystallized, and a crystallographic data set was collected. The crystals belonged to the monoclinic space group <italic>C</italic>2, with unit‐cell parameters <italic>a</italic> = 79.2, <italic>b</italic> = 69.9, <italic>c</italic> = 57.9 Å, β = 129.3°, contained one monomer per asymmetric unit and diffracted to a resolution of 1.98 Å.</p> </abstract>