Effect of melatonin treatment on developmental potential of somatic cell nuclear-transferred mouse oocytes in vitro. Issue 2 (May 2014)

• Record Type: Journal Article
• Authors: Salehi, Mohammad; Kato, Yoko; Tsunoda, Yukio
• Is Part Of: Zygote. Volume 22:Issue 2(2014)
• Page Start: 213
• Page End: 217
• Publication Date: 2014-05
• Subjects: Developmental biology -- Periodicals; Embryology -- Periodicals; Zygotes -- Periodicals; Gametes -- Periodicals; 571.864
• DOI: 10.1017/S0967199413000336
• Languages: English
• ISSNs: 0967-1994

Abstract

<abstract abstract-type="normal"> <title>Summary</title> <p>The beneficial effect of supplementing culture medium with melatonin has been reported during <italic>in vitro</italic> embryo development of species such as mouse, bovine and porcine. However, the effect of melatonin on mouse somatic cell nuclear transfer remains unknown. In this study, we assessed the effects of various concentrations of melatonin (10⁻⁶ to 10⁻¹² M) on the <italic>in vitro</italic> development of mouse somatic cell nuclear transfer embryos for 96 h. Embryos cultured without melatonin were used as control. There was no significant difference in cleavage rates between the groups supplemented with melatonin, dimethyl sulphoxide (DMSO) and the control. The rate of development to blastocyst stage was significantly higher in the group supplemented with 10⁻¹² M melatonin compared with the control group (<italic>P</italic> &lt; 0.05). Thus, our data demonstrated that adding melatonin to pre-implantation mouse nuclear-transferred embryos can accelerate blastocyst formation.</p> </abstract>