Relationship between serum reactive oxidative metabolite level and skin reaction in an irradiated rat model. (May 2014)

• Record Type: Journal Article
• Authors: Nomiya, Takuma; Kaneko, Takashi; Goto, Jun; Harada, Mayumi; Akamatsu, Hiroko; Hagiwara, Yasuhito; Ota, Ibuki; Nemoto, Kenji
• Is Part Of: Free radical research. Volume 48:Number 5(2014:May)
• Page Start: 572
• Page End: 579
• Publication Date: 2014-05
• Subjects: Free radicals (Chemistry) -- Periodicals; Antioxidants -- Periodicals; Vitamin C -- Periodicals; Vitamin E -- Periodicals; 541.224
• DOI: 10.3109/10715762.2014.894637
• Languages: English
• ISSNs: 1071-5762

Abstract

<abstract> <title>Abstract</title> <p> <italic>Purpose.</italic> Ionizing radiation generates free radicals and reactive oxygen species that induce DNA damage <italic>in vivo</italic>. This study aimed to determine the relationship between serum reactive oxygen metabolite (ROM) levels and skin reaction after irradiation in a rat model. <italic>Methods and materials.</italic> I. Female Wistar rats were classified into 0 Gy (control), 2 Gy, and 30 Gy groups; serum ROM levels were measured in the very acute phase. II. Other female Wistar rats were classified into 0 Gy (control), 30 Gy, 50 Gy, and 70 Gy groups; serum ROM levels were measured before and 3, 7, 16, 24, 31, and 38 days after irradiation. Skin reaction was evaluated according to the SRS (0–5) twice every week. <italic>Results.</italic> Serum ROM levels in the subacute phase were significantly higher in the 50 and 70 Gy groups than in the 0 and 30 Gy groups [<italic>p</italic> = 0.029, repeated-measure analysis of variance (ANOVA)]. As expected, SRSs increased in the order of the 0 Gy, 30 Gy, 50 Gy, and 70 Gy groups and differed significantly among these groups (<italic>p</italic> < 0.001, repeated-measure ANOVA). Peak serum ROM levels were observed 16 days after irradiation in all irradiated groups and corresponded with the appearance of visible skin reaction after irradiation. <italic>Conclusions.</italic> Serum ROM levels may be useful for evaluating radiation damage in mammals. Further investigations are required to investigate changes in intracellular metabolism after irradiation at gene and protein levels.</p> </abstract>