Differentiation of human neural progenitor cell-derived spiral ganglion-like neurons: a time-lapse video study. (May 2014)

• Record Type: Journal Article
• Authors: Edin, Fredrik; Liu, Wei; Boström, Marja; Magnusson, Peetra U.; Rask-Andersen, Helge
• Is Part Of: Acta oto-laryngologica. Volume 134:Number 5(2014)
• Page Start: 441
• Page End: 447
• Publication Date: 2014-05
• Subjects: Otolaryngology -- Periodicals; Ear -- Diseases -- Periodicals; Throat -- Diseases -- Periodicals; Otolaryngology -- Electronic Resources; Otorhinolaryngologic Diseases; 617.8
• DOI: 10.3109/00016489.2013.875220
• Languages: English
• ISSNs: 0001-6489

Abstract

<abstract> <title>Abstract</title> <p> <italic>Conclusions:</italic> Human neural progenitor cells can differentiate into spiral ganglion-like cells when exposed to inner ear-associated growth factors. The phenotype bears resemblance to human sphere-derived neurons. <italic>Objective:</italic> To establish an <italic>in vitro</italic> model for the human auditory nerve to replace and complement <italic>in vivo</italic> animal experiments and ultimately human <italic>in vivo</italic> transplantation. <italic>Methods:</italic> Human neural progenitors were differentiated under conditions developed for <italic>in vitro</italic> survival of human primary spiral ganglion culture with media containing growth factors associated with inner ear development. Differentiation was documented using time-lapse video microscopy. Time-dependent marker expression was evaluated using immunocytochemistry with fluorescence and laser confocal microscopy. <italic>Results:</italic> Within 14 days of differentiation, neural progenitors adopted neural phenotype and expressed spiral ganglion-associated markers.</p> </abstract>