Anti‐biofilm and antibacterial activities of zinc oxide nanoparticles against the oral opportunistic pathogens Rothia dentocariosa and Rothia mucilaginosa. (14th October 2014)

• Record Type: Journal Article
• Authors: Khan, Shams T.; Ahamed, Maqusood; Musarrat, Javed; Al‐Khedhairy, Abdulaziz A.
• Is Part Of: European journal of oral sciences. Volume 122:Number 6(2014:Dec.)
• Page Start: 397
• Page End: 403
• Publication Date: 2014-10-14
• Subjects: Dentistry -- Periodicals; Oral medicine -- Periodicals; 617.6005
• DOI: 10.1111/eos.12152
• Languages: English
• ISSNs: 0909-8836

Abstract

<abstract abstract-type="main" id="eos12152-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <p>Species of the genus <italic>Rothia</italic> that inhabit the oral cavity have recently been implicated in a number of diseases. To minimize their role in oral infections, it is imperative to reduce and/or control the growth and biofilm formation activity of <italic>Rothia</italic> spp. In this study, two bacterial isolates, Ora‐7 and Ora‐16, were obtained from the oral cavity of a healthy male subject and identified as <italic>Rothia dentocariosa</italic> and <italic>Rothia mucilaginosa</italic>, respectively, using a polyphasic taxonomic approach. Antimicrobial and anti‐biofilm formation activities of zinc oxide nanoparticles (ZnO‐NPs), of average size 35 nm, were assessed in <italic>in vitro</italic> assays using Crystal Violet and live and dead staining techniques. The ZnO‐NPs exhibited an IC₅₀ value of 53 and 76 <italic>μ</italic>g ml⁻¹ against <italic>R. dentocariosa</italic> and <italic>R. mucilaginosa</italic>, respectively. Biofilm‐formation assays, performed on the surfaces of polystyrene plates, artificial teeth, and dental prostheses, revealed the efficacy of ZnO‐NPs as a potential antibacterial agent for controlling the growth of <italic>Rothia</italic> isolates in both planktonic form and biofilm.</p> </abstract>