Enrichment of LAG‐3, but not PD‐1, on Double Negative T Cells at the Female Genital Tract. (25th August 2014)

• Record Type: Journal Article
• Authors: Juno, Jennifer A.; Lajoie, Julie; Stalker, Andrew T.; Oyugi, Julius; Kimani, Makobu; Kimani, Joshua; Plummer, Francis A.; Fowke, Keith R.
• Is Part Of: American journal of reproductive immunology. Volume 72:Number 6(2014:Dec.)
• Page Start: 534
• Page End: 540
• Publication Date: 2014-08-25
• Subjects: Human reproduction -- Immunological aspects -- Periodicals; 616.69206
• DOI: 10.1111/aji.12308
• Languages: English
• ISSNs: 1046-7408

Abstract

<abstract abstract-type="main" id="aji12308-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="aji12308-sec-0001" sec-type="section"> <title>Problem</title> <p>The expression of inhibitory markers such as LAG‐3 and PD‐1 on T lymphocytes regulates immune function. Their expression at the genital mucosa is poorly understood, but regulation of immune activation at the female genital tract likely controls susceptibility to sexually transmitted infections.</p> </sec> <sec id="aji12308-sec-0002" sec-type="section"> <title>Method of study</title> <p>Cervical mononuclear cells were phenotyped by flow cytometry. Concentrations of cytokines were determined in cervical‐vaginal lavage samples by bead array.</p> </sec> <sec id="aji12308-sec-0003" sec-type="section"> <title>Results</title> <p>LAG‐3 expression was significantly elevated at the genital mucosa and was associated with expression of CCR5 and CD69. Double negative (DN) T cells expressed the highest levels of LAG‐3, but not PD‐1, and were more activated than other T lymphocytes.</p> </sec> <sec id="aji12308-sec-0004" sec-type="section"> <title>Conclusion</title> <p>The elevated expression of LAG‐3 at the genital tract suggests it may regulate T‐cell activation, and identify cells susceptible to HIV infection. The enrichment of LAG‐3 on DN T cells suggests LAG‐3 may contribute to the immunoregulatory activity of these cells.</p> </sec> </abstract>