Hydrogen sulphide increases hepatic differentiation of human tooth pulp stem cells compared with human bone marrow stem cells. (20th March 2014)

• Record Type: Journal Article
• Authors: Okada, M.; Ishkitiev, N.; Yaegaki, K.; Imai, T.; Tanaka, T.; Fukuda, M.; Ono, S.; Haapasalo, M.
• Is Part Of: International endontic journal. Volume 47:Number 12(2014:Dec.)
• Page Start: 1142
• Page End: 1150
• Publication Date: 2014-03-20
• Subjects: Endodontics -- Periodicals; 617.6342
• DOI: 10.1111/iej.12262
• Languages: English
• ISSNs: 0143-2885

Abstract

<abstract abstract-type="main" id="iej12262-abs-0001"> <title>Abstract</title> <sec id="iej12262-sec-0001" sec-type="section"> <title>Aim</title> <p>To determine the differences in stem cell properties, in hepatic differentiation and in the effects of hydrogen sulphide (H<sub>2</sub>S) on hepatic differentiation between human bone marrow stem cells (hBMC) and stem cells from human exfoliated primary tooth pulp (SHED).</p> </sec> <sec id="iej12262-sec-0002" sec-type="section"> <title>Methodology</title> <p>CD117<sup>+</sup> cells were magnetically separated and subjected to hepatic differentiation. CD117<sup>+</sup> cell lineages were characterized for transcription factors indicative of stem cells by qRT‐PCR. For the last 9 days of the differentiation, the test cells were exposed to 0.1 ng mL<sup>−1</sup> H<sub>2</sub>S. Immunocytochemistry and flow cytometry of albumin, alpha‐fetoprotein and carbamoyl phosphate synthetase were carried out after differentiation. Urea concentration and glycogen synthesis were also determined.</p> </sec> <sec id="iej12262-sec-0003" sec-type="section"> <title>Results</title> <p>Genes expressed in SHED were also expressed in BMC. No difference in expression level of hepatic markers was shown by immunofluorescence. SHED showed more positive cells than hBMC (<italic>P</italic> < 0.01). H<sub>2</sub>S increased the number of positive cells in both cultures (<italic>P</italic> < 0.01). Urea concentration and glycogen synthesis increased significantly after H<sub>2</sub>S exposure (<italic>P</italic> < 0.001 and <italic>P</italic> < 0.05, respectively). Real‐time PCR data were analysed by RT<sup>2</sup> profiler RT‐PCR Array Data Analysis version 3.5 (Qiagen), and ELISA data were analysed by Bonferroni's multiple comparison using Windows <sc>spss</sc> version 16 (SPSS Inc, Chicago, IL, USA). Bonferroni's multiple comparison test was also carried out after angle transformation for the percentage data of flow cytometer using Windows <sc>spss</sc><sup>®</sup> version 16 (SPSS Inc). Statistical significance was accepted at <italic>P</italic> < 0.05.</p> </sec> <sec id="iej12262-sec-0004" sec-type="section"> <title>Conclusions</title> <p>Stem cells from human exfoliated primary tooth pulp and BMC have similar properties. The level of hepatic differentiation in SHED compared with BMC was the same or higher. H<sub>2</sub>S increased the level of hepatic differentiation.</p> </sec> </abstract>