Unsupervised cell identification on multidimensional X‐ray fluorescence datasets. (17th March 2014)

• Record Type: Journal Article
• Authors: Wang, Siwei; Ward, Jesse; Leyffer, Sven; Wild, Stefan M.; Jacobsen, Chris; Vogt, Stefan
• Is Part Of: Journal of synchrotron radiation. Volume 21:Part 3(2014)
• Page Start: 568
• Page End: 579
• Publication Date: 2014-03-17
• Subjects: Synchrotron radiation -- Periodicals; Free electron lasers -- Periodicals; 539.73505
• DOI: 10.1107/S1600577514001416
• Languages: English
• ISSNs: 0909-0495

Abstract

<abstract abstract-type="main" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p>A novel approach to locate, identify and refine positions and whole areas of cell structures based on elemental contents measured by X‐ray fluorescence microscopy is introduced. It is shown that, by initializing with only a handful of prototypical cell regions, this approach can obtain consistent identification of whole cells, even when cells are overlapping, without training by explicit annotation. It is robust both to different measurements on the same sample and to different initializations. This effort provides a versatile framework to identify targeted cellular structures from datasets too complex for manual analysis, like most X‐ray fluorescence microscopy data. Possible future extensions are also discussed.</p> </abstract>