Looking at proteins from two dimensions: a review on five decades of 2D electrophoresis. (December 2014)

• Record Type: Journal Article
• Authors: Westermeier, Reiner
• Is Part Of: Archives of physiology and biochemistry. Volume 120:Number 5(2014)
• Page Start: 168
• Page End: 172
• Publication Date: 2014-12
• Subjects: Physiology -- Periodicals; Biochemistry -- Periodicals; Biophysics -- Periodicals; Biochemistry; Physiology; 571
• DOI: 10.3109/13813455.2014.945188
• Languages: English
• ISSNs: 1381-3455

Abstract

<abstract> <title>Abstract</title> <p>Separating proteins according to two different gel-electrophoretic methods not only increases the resolution power for highly complex samples when compared to one-dimensional separations, but is also a valuable tool for protein and protein complex characterization. There are a number of different electrophoresis methods which can be combined. The combination of isoelectric focusing under denaturing conditions and SDS polyacrylamide gel electrophoresis delivers the highest resolution of all bio-analytic techniques. This is a short review on the history and state of the art of two-dimensional electrophoresis methods, and contains some practical tips for high resolution 2D electrophoresis, which are based on several decades of experience with this method.</p> </abstract>